去硫酸化牛纤维蛋白肽B的制备及其在体外由神经母细胞瘤-胶质瘤杂交细胞的酶系统进行硫酸化的证明。

Preparation of desulphated bovine fibrinopeptide B and demonstration of its sulphation in vitro by an enzyme system from neuroblastoma-glioma hybrid cells.

作者信息

Barling P M, Palmer D J, Christie D L

出版信息

Int J Biochem. 1986;18(2):137-41. doi: 10.1016/0020-711x(86)90145-x.

Abstract

A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the product with acid. After incubating DSbFPB with [35S]PAPS, in the presence of a particulate preparation from neuroblastoma-glioma hybrid cells, radioactivity was incorporated into a product identified as [35S]bFPB from its position of elution on reverse-phase HPLC. The possible significance of this observation is discussed.

摘要

本文描述了一种使用DEAE纤维素离子交换色谱法从粗制牛纤维蛋白原中以高纯度形式分离牛纤维蛋白肽B（bFPB）的方法。通过用酸处理产物制备了去硫酸化的bFPB（称为DSbFPB）。在神经母细胞瘤-胶质瘤杂交细胞的颗粒制剂存在下，将DSbFPB与[35S]PAPS一起孵育后，放射性被掺入到一种产物中，根据其在反相HPLC上的洗脱位置，该产物被鉴定为[35S]bFPB。讨论了这一观察结果的可能意义。