Baker M A, Kanani A, Hindenburg A, Taub R N
Br J Haematol. 1986 Mar;62(3):431-8. doi: 10.1111/j.1365-2141.1986.tb02954.x.
Granulocytes from patients with chronic myelogenous leukaemia (CML) have been previously shown to have aberrant sialylation of membrane glycoproteins. We have examined the granulocytes from CML patients receiving intermittent chemotherapy to determine the relationship of the oligosaccharide changes to treatment. Compared to cells from non-leukaemic patients, granulocytes from untreated CML patients showed less adherence to nylon wool, decreased reactivity with peanut lectin, and decreased binding of the synthetic chemotactic peptide formyl-methionine-leucine-phenylalanine (FMLP). Granulocytes from CML patients treated with chemotherapy showed nylon wool adherence, peanut lectin reactivity and FMLP binding comparable to non-leukaemic cells. Chemotherapeutic agents may interfere with oligosaccharide synthesis with a resulting change in the composition of cell surface glycoconjugates.
慢性粒细胞白血病（CML）患者的粒细胞先前已被证明存在膜糖蛋白异常唾液酸化。我们检测了接受间歇性化疗的CML患者的粒细胞，以确定寡糖变化与治疗的关系。与非白血病患者的细胞相比，未经治疗的CML患者的粒细胞对尼龙毛的黏附性降低、与花生凝集素的反应性降低，以及对合成趋化肽甲酰甲硫氨酸-亮氨酸-苯丙氨酸（FMLP）的结合减少。接受化疗的CML患者的粒细胞表现出与非白血病细胞相当的尼龙毛黏附性、花生凝集素反应性和FMLP结合。化疗药物可能会干扰寡糖合成，从而导致细胞表面糖缀合物组成的变化。
