物种的基因组分析揭示了展青霉素和桔霉素基因簇以及参与氧脂素产生的新位点。

Genomic Analyses of Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production.

作者信息

Yin Guohua, Zhao Hui, Pennerman Kayla K, Jurick Wayne M, Fu Maojie, Bu Lijing, Guo Anping, Bennett Joan W

机构信息

Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.

Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.

出版信息

J Fungi (Basel). 2021 Sep 9;7(9):743. doi: 10.3390/jof7090743.

DOI:10.3390/jof7090743

PMID:34575780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8464941/

Abstract

Blue mold of apple is caused by several different species, among which and are the most frequently isolated. is the most aggressive species, and is very weak when infecting apple fruit during storage. In this study, we report complete genomic analyses of three different species: R21 and NJ1, isolated from stored apple fruit; and . 113, isolated in 2013 from a flooded home in New Jersey, USA, in the aftermath of Hurricane Sandy. Patulin and citrinin gene cluster analyses explained the lack of patulin production in NJ1 compared to R21 and lack of citrinin production in all three strains. A bioassay demonstrated that volatiles emitted by . SA and . RS1 were more toxic than those from . and strains (R27, R11, R21, G10, and R19). The toxicity was hypothesized to be related to production of eight-carbon oxylipins. Putative lipoxygenase genes were identified in . and . strains, but not in . . Our data will provide a better understanding of spp. complex secondary metabolic capabilities, especially concerning the genetic bases of mycotoxins and toxic VOCs.

摘要

苹果青霉病由几种不同的物种引起，其中和是最常分离到的。是最具侵袭性的物种，而在侵染贮藏期苹果果实方面非常弱。在本研究中，我们报告了三种不同物种的全基因组分析：从贮藏苹果果实中分离出的 R21 和 NJ1；以及2013年在美国新泽西州桑迪飓风过后从一所被洪水淹没的房屋中分离出的 113。展青霉素和桔霉素基因簇分析解释了与 R21 相比 NJ1 中展青霉素产量缺乏以及所有三个菌株中桔霉素产量缺乏的原因。一项生物测定表明， SA 和 RS1 释放的挥发物比以及菌株（R27、R11、R21、G10 和 R19）释放的挥发物毒性更大。据推测，毒性与八碳氧脂素的产生有关。在和菌株中鉴定出了假定的脂氧合酶基因，但在中未鉴定出。我们的数据将有助于更好地理解物种复合体的次生代谢能力，特别是关于霉菌毒素和有毒挥发性有机化合物的遗传基础。