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DPY19L3 参与 C2C12 成肌细胞的肌发生分化。

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts.

机构信息

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.

出版信息

Molecules. 2021 Sep 19;26(18):5685. doi: 10.3390/molecules26185685.

DOI:10.3390/molecules26185685
PMID:34577156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8467457/
Abstract

DPY19L3 has been identified as a -mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated -mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

摘要

DPY19L3 被鉴定为一种血栓反应素型-1 重复结构域蛋白的 -甘露糖基转移酶。在本研究中，我们专注于 DPY19L3 在 C2C12 小鼠成肌细胞的成肌分化中的作用。我们使用 CRISPR/Cas9 系统进行 DPY19L3 基因缺失。结果表明，这些 DPY19L3 敲除细胞不能被诱导分化。此外，与亲本细胞相比，DPY19L3 敲除细胞中 MEK/ERK 和 p70S6K 的磷酸化水平受到抑制，这表明 DPY19L3 介导的 -甘露糖化并调节 MEK/ERK 或 p70S6K 信号的蛋白对于分化是必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/6210acb689c9/molecules-26-05685-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/7401fd325a36/molecules-26-05685-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/1eb3a42b0b00/molecules-26-05685-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/d9105f920a2c/molecules-26-05685-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/6210acb689c9/molecules-26-05685-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/7401fd325a36/molecules-26-05685-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/1eb3a42b0b00/molecules-26-05685-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/d9105f920a2c/molecules-26-05685-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/8467457/6210acb689c9/molecules-26-05685-g004.jpg

相似文献

[1]
Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts.

Molecules. 2021-9-19

[2]
Estrogen-related receptor α regulates skeletal myocyte differentiation via modulation of the ERK MAP kinase pathway.

Am J Physiol Cell Physiol. 2011-5-11

[3]
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells.

Mol Biol Cell. 2016-3-1

[4]
DPY19L3 promotes vasculogenic mimicry by its -mannosyltransferase activity.

Oncol Res. 2024

[5]
Creatine enhances differentiation of myogenic C2C12 cells by activating both p38 and Akt/PKB pathways.

Am J Physiol Cell Physiol. 2007-10

[6]
Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation.

Int J Mol Med. 2016-11

[7]
The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development.

Mol Cell Proteomics. 2021

[8]
Exposure to TNF-alpha but not IL-1beta impairs insulin-dependent phosphorylation of protein kinase B and p70S6k in mouse C2C12 myogenic cells.

Pol J Vet Sci. 2006

[9]
Treatment with IFN-gamma prevents insulin-dependent PKB, p70S6k phosphorylation and protein synthesis in mouse C2C12 myogenic cells.

Cell Biol Int. 2009-12-16

[10]
The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.

Biochem Biophys Res Commun. 2016-9-23

引用本文的文献

[1]
Protective Efficacy of Postbiotic beLP-K in a Dexamethasone-Induced Sarcopenia Model.

Int J Mol Sci. 2025-8-3

[2]
Enhanced Osteogenic Potential of Knockout C2C12 Cells on BMP-2 Releasing Silk Scaffolds.

ACS Biomater Sci Eng. 2023-11-13

[3]
Correction: Mori et al. Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts. 2021, , 5685.

Molecules. 2022-5-31

本文引用的文献

[1]
Regulation of N-glycosylation and secretion of Isthmin-1 by its C-mannosylation.

Biochim Biophys Acta Gen Subj. 2021-3

[2]
Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4).

Biochim Biophys Acta Gen Subj. 2021-3

[3]
Lmod3 promotes myoblast differentiation and proliferation via the AKT and ERK pathways.

Exp Cell Res. 2020-11-15

[4]
O-GlcNAcylation of Mef2c regulates myoblast differentiation.

Biochem Biophys Res Commun. 2020-8-27

[5]
The fibrinogen C-terminal domain is seldom C-mannosylated but its C-mannosylation is important for the secretion of microfibril-associated glycoprotein 4.

Biochim Biophys Acta Gen Subj. 2020-5-19

[6]
Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1).

Biochem Biophys Rep. 2020-1-30

[7]
C‑mannosylation of R‑spondin2 activates Wnt/β‑catenin signaling and migration activity in human tumor cells.

Int J Oncol. 2019-4-1

[8]
Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells.

Cancer Sci. 2018-7-9

[9]
Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation.

Biochem Biophys Res Commun. 2018-4-6

[10]
Lactate increases myotube diameter via activation of MEK/ERK pathway in C2C12 cells.

Acta Physiol (Oxf). 2018-2-9

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