Lukianova Anna A, Evseev Peter V, Stakheev Alexander A, Kotova Irina B, Zavriev Sergey K, Ignatov Alexander N, Miroshnikov Konstantin A
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia.
Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, Bldg. 12, 119234 Moscow, Russia.
Plants (Basel). 2021 Sep 10;10(9):1880. doi: 10.3390/plants10091880.
is a plant-pathogenic bacterium, recently attributed as a separate species, which infects potatoes, causing soft rot in tubers. The distribution of seems to be global, although the bacterium tends to be accommodated to moderate climates. Fast and accurate detection systems for this pathogen are needed to study its biology and to identify latent infection in potatoes and other plant hosts. The current paper reports on the development of a specific and sensitive detection protocol based on a real-time PCR with a TaqMan probe for , and its evaluation. In sensitivity assays, the detection threshold of this protocol was 10 cfu/mL on pure bacterial cultures and 10-10 cfu/mL on plant material. The specificity of the protocol was evaluated against and more than 100 strains of potato-associated species of and . No cross-reaction with the non-target bacterial species, or loss of sensitivity, was observed. This specific and sensitive diagnostic tool may reveal a wider distribution and host range for and will expand knowledge of the life cycle and environmental preferences of this pathogen.
是一种植物病原细菌，最近被归为一个独立的物种，它感染马铃薯，导致块茎软腐。尽管这种细菌倾向于适应温和气候，但其分布似乎是全球性的。需要快速准确的检测系统来研究其生物学特性，并识别马铃薯和其他植物宿主中的潜伏感染。本文报道了一种基于实时PCR和TaqMan探针的针对该细菌的特异性和灵敏检测方案的开发及其评估。在灵敏度测定中，该方案在纯细菌培养物上的检测阈值为10 cfu/mL，在植物材料上为10 - 10 cfu/mL。该方案的特异性是针对该细菌以及100多种与马铃薯相关的该细菌和其他物种的菌株进行评估的。未观察到与非靶标细菌物种的交叉反应或灵敏度丧失。这种特异性和灵敏的诊断工具可能揭示该细菌更广泛的分布和宿主范围，并将扩展对该病原体生命周期和环境偏好的认识。
