EVA71 和 CVA16 共同免疫诱导产生针对四价肠道病毒单价疫苗的广谱且强效的 IgM 抗体。

A broad and potent IgM antibody against tetra-EV-As induced by EVA71 and CVA16 co-immunization.

机构信息

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.

出版信息

Vaccine. 2021 Oct 22;39(44):6510-6519. doi: 10.1016/j.vaccine.2021.09.056. Epub 2021 Sep 29.

DOI:10.1016/j.vaccine.2021.09.056

PMID:34600750

Abstract

OBJECTIVE

To determine the potent and broad neutralizing monoclonal antibody (mAb) against enterovirus A (EV-A) in vitro and in vivo induced by enterovirus A71(EVA71) and coxsackievirus 16 (CVA16) co-immunization.

METHODS

The mAb was Generated by co-immunization with EVA71 and CVA16 through hybridomas technology. The characteristics and neutralizing ability of mAb were analysed in vitro and in mice.

RESULTS

We screened three mAb, the IgM antibody M20 and IgG antibody B1 and C31. All three antibodies showed cross-reactivity against tetra-EV-As. However, M20 showed potent and broad neutralizing ability against tetra-EV-As than B1 and C31. Meanwhile, M20 provided cross-antiviral efficacy in tetra-EV-As orally infected mice. Moreover, M20 binds to a conserved neutralizing epitope within the GH loop of tetra-EV-As VP1.

CONCLUSIONS

M20 and its property exhibited potent and broad antiviral activity against tetra-EV-As, and that is expected to be a potential preventive and therapeutic candidate against EV-As.

摘要

目的

在体外和体内确定由肠道病毒 A71（EVA71）和柯萨奇病毒 16（CVA16）共同免疫诱导的针对肠道病毒 A（EV-A）的有效且广谱的中和单克隆抗体（mAb）。

方法

通过杂交瘤技术，用 EVA71 和 CVA16 共同免疫产生 mAb。分析 mAb 的特性和中和能力。

结果

我们筛选出了三种 mAb，即 IgM 抗体 M20 和 IgG 抗体 B1 和 C31。这三种抗体均表现出对四价肠道病毒的交叉反应性。然而，M20 对四价肠道病毒的中和能力比 B1 和 C31 更强。同时，M20 为口服感染四价肠道病毒的小鼠提供了交叉抗病毒功效。此外，M20 与四价肠道病毒 VP1 的 GH 环内的保守中和表位结合。