Passos Larissa Souza, Coppo Gabriel Carvalho, Pereira Tatiana Miura, Teixeira Barbara Chisté, Bona Alliny Magalhães, Merçon Julia, Lopes Taciana Onesorge Miranda, Chippari-Gomes Adriana Regina
Laboratory of Applied Ichthyology, Complex of Biopractices, Vila Velha University, Rua José Dantas de Melo, 21, Boa Vista, Vila Velha, ES, CEP 29102-770, Brazil.
Laboratory of Toxins and Natural Algae Products, Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bl. 17, CEP 05508-000, São Paulo, SP, Brazil.
Bull Environ Contam Toxicol. 2022 Apr;108(4):708-715. doi: 10.1007/s00128-021-03382-6. Epub 2021 Oct 9.
The present study aimed to evaluate the toxicity of the association between Fe and Mn in Oreochromis niloticus through genotoxic (micronucleus test and comet assay) and biochemical (CAT and GST enzymes) assays. The tested treatments were T1 = control group (without metal addition), T2 = 2.60 mg L of Fe + 0.2 mg L of Mn, and T3 = 4.40 mg L of Fe + 3.49 mg L of Mn, during 96-h bioassays. All animals exposed to the metals showed a significant increase in erythrocyte micronucleus frequency and DNA damage. The hepatic GST activity increased two times in animals exposed to T3 compared to the control group. The results indicate that Fe + Mn caused genotoxic and biochemical changes in exposed fish. Therefore, excess metals in ecosystems, even those essential for organisms, can be dangerous for the local biota due to the risk associated with high concentrations of these metals.
本研究旨在通过遗传毒性（微核试验和彗星试验）和生化（CAT和GST酶）试验评估尼罗罗非鱼中Fe和Mn联合作用的毒性。在96小时生物测定期间，测试处理为T1 =对照组（不添加金属），T2 = 2.60 mg/L的Fe + 0.2 mg/L的Mn，以及T3 = 4.40 mg/L的Fe + 3.49 mg/L的Mn。所有暴露于金属的动物红细胞微核频率和DNA损伤均显著增加。与对照组相比，暴露于T3的动物肝脏GST活性增加了两倍。结果表明，Fe + Mn对暴露的鱼类造成了遗传毒性和生化变化。因此，生态系统中过量的金属，即使是生物体必需的金属，由于与这些金属高浓度相关的风险，也可能对当地生物群造成危险。
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