Department of Clinical Biochemistry, Sivas Numune State Hospital, Ministry of Health, Sivas, Turkey.
Department of Clinical Biochemistry, Ankara Training and Research Hospital, Ministry of Health Ankara, Turkey.
Int J Clin Pract. 2021 Dec;75(12):e14969. doi: 10.1111/ijcp.14969. Epub 2021 Oct 21.
Obesity and overweight are significant public health problems because of higher risk for coronary artery disease (CAD). It is very important to determine new predictive markers to identify the CAD risk in obese and overweight. To aim this, we analysed HDL-C subgroups (HDL2-C and HDL3-C) and their paraoxonase-1 (PON-1) activity in obese, overweight and normal weight subjects.
71 obese, 40 overweight and 30 healthy subjects as a control group were enrolled the study. Serum lipids levels were determined with enzymatic colorimetric method. Further, PON-1 activities and HDL-C levels were determined by spectrophotometric methods. Non-HDL3-C concentrations were calculated with the subtraction of HDL3-C from total HDL-C.
The mean serum levels of total HDL-C, HDL3-C, Non-HDL3-C and ApoA1 were higher in control group than obese and overweight groups. There were a statistically significant difference between obese and control group in terms of Lp(a), hsCRP and HOMA index. Higher total PON-1, non-HDL3 PON-1 and HDL3 PON-1 activities were found in the control group compared with obese and overweight groups. Total HDL was weakly negative correlated with the HOMA index, BMI and waist circumference. There was a weak negative correlation between non-HDL3-C and waist circumference.
Altered HDL-subgroups pattern and decreased PON-1 activities may cause increased risk for CVD in obese and overweight individuals. Therefore determination of HDL subgroups and their PON-1 activity may improve risk prediction compared with measuring total HDL-C levels and its PON-1 activity alone. Body weight and insulin resistance appear to have a role in the decreased HDL-C levels and PON-1activity in obese. Further studies should be conducted to shed more light on impacts of these markers in CVD.
肥胖和超重是重大的公共卫生问题，因为它们会增加患冠状动脉疾病（CAD）的风险。确定新的预测标志物来识别肥胖和超重人群中的 CAD 风险非常重要。为此，我们分析了高密度脂蛋白亚组（HDL2-C 和 HDL3-C）及其对氧磷酶-1（PON-1）活性在肥胖、超重和正常体重人群中的变化。
本研究纳入了 71 名肥胖者、40 名超重者和 30 名健康对照组。采用酶比色法检测血清脂质水平。进一步采用分光光度法检测 PON-1 活性和 HDL-C 水平。非 HDL3-C 浓度通过从总 HDL-C 中减去 HDL3-C 来计算。
与肥胖和超重组相比，对照组的总 HDL-C、HDL3-C、非 HDL3-C 和 ApoA1 血清水平更高。肥胖组与对照组在 Lp(a)、hsCRP 和 HOMA 指数方面存在统计学差异。对照组的总 PON-1、非 HDL3-PON-1 和 HDL3-PON-1 活性均高于肥胖和超重组。总 HDL 与 HOMA 指数、BMI 和腰围呈弱负相关。非 HDL3-C 与腰围呈弱负相关。
HDL 亚群模式改变和 PON-1 活性降低可能导致肥胖和超重人群患 CVD 的风险增加。因此，与单独测量总 HDL-C 水平及其 PON-1 活性相比，测定 HDL 亚群及其 PON-1 活性可能会改善风险预测。体重和胰岛素抵抗可能在肥胖人群中 HDL-C 水平和 PON-1 活性降低中起作用。应进一步开展研究，以阐明这些标志物在 CVD 中的作用。
