College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Hanwoo Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang, Korea.
Reprod Domest Anim. 2022 Jan;57(1):45-54. doi: 10.1111/rda.14027. Epub 2021 Oct 19.
We aimed to evaluate whether a modified pre-synchronization-Ovsynch (MPO) and resynchronization method, adjusted for the timing of biweekly reproductive consulting visits, could be implemented successfully under field conditions in dairy herds. Forty-five days after calving (calving: Day 0), 1,182 cows were assigned randomly to Ovsynch, MPO or control groups. (1) Cows were administered GnRH on Day 65, PGF on Day 72 and GnRH 56 hr later, followed by timed artificial insemination (TAI) 16 hr later (Ovsynch, n = 380). (2) Cows with a CL were administered PGF (n = 204) and cows without were administered GnRH (n = 199) on Day 45. Then, each cow was administered PGF on Day 55 (9 to 12 days later), GnRH on Day 58 and underwent Ovsynch 7 days later (selective MPO [sMPO] n = 403). (3) The remaining cows underwent AI when oestrus was detected (EDAI, n = 399). The probabilities of pregnancy per AI 30 and 45 days after the first AI were higher in the sMPO group (odds ratios 1.99 and 1.92, p < .0001) than in the EDAI group. In addition, the probability of pregnancy by 105 days postpartum tended to be higher (p < .1) in the sMPO group than in the EDAI and Ovsynch groups, which shortened the mean interval from calving to pregnancy in the sMPO group (3.4 and 4.2 days, respectively) than in the EDAI and Ovsynch groups. In the sMPO group, the interval between the first and second treatments during pre-synchronization did not affect the probability of pregnancy per AI after the first AI. The probability of pregnancy per AI after the second AI did not differ between the Ovsynch, sMPO and EDAI groups. In conclusion, the sMPO programme, adjusted for the timing of biweekly reproductive consulting visits, followed by resynchronization, could be successfully adopted in field conditions, and is associated with good reproductive performance in dairy herds.
我们旨在评估一种改良的预同期-Ovsynch(MPO)和再同步方法,该方法根据每两周进行一次生殖咨询访问的时间进行调整,是否可以在奶牛场的田间条件下成功实施。产后 45 天(产后:第 0 天),1182 头奶牛被随机分配到 Ovsynch、MPO 或对照组。(1)奶牛在第 65 天接受 GnRH,第 72 天接受 PGF,56 小时后再次接受 GnRH,然后 16 小时后进行定时人工授精(TAI)(Ovsynch,n=380)。(2)有 CL 的奶牛在第 45 天接受 PGF(n=204),没有 CL 的奶牛接受 GnRH(n=199)。然后,每头奶牛在第 55 天接受 PGF(9 到 12 天后),第 58 天接受 GnRH,并在 7 天后进行 Ovsynch(选择性 MPO[sMPO],n=403)。(3)其余奶牛在发情时接受人工授精(EDAI,n=399)。第一次 AI 后 30 天和 45 天的妊娠概率在 sMPO 组更高(优势比 1.99 和 1.92,p<0.0001),而在 EDAI 组则较低。此外,sMPO 组产后 105 天的妊娠概率趋于更高(p<0.1),sMPO 组从产犊到妊娠的平均间隔缩短(分别为 3.4 和 4.2 天),而 EDAI 和 Ovsynch 组则更长。在 sMPO 组中,预同步期间第一次和第二次治疗之间的间隔不会影响第一次 AI 后每次 AI 的妊娠概率。第二次 AI 后每次 AI 的妊娠概率在 Ovsynch、sMPO 和 EDAI 组之间没有差异。总之,调整了每两周进行一次生殖咨询访问的时间的 sMPO 方案,随后进行再同步,可以在田间条件下成功采用,并与奶牛场的良好繁殖性能相关。