Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, PR China.
Int J Biol Macromol. 2021 Dec 1;192:557-563. doi: 10.1016/j.ijbiomac.2021.10.024. Epub 2021 Oct 12.
The high-amylopectin glutinous rice is used in this study for the preparation of starch nanocrystals (SNCs) with the acid hydrolysis and enzymatic treatment. The fabricated SNC is carried out the surface modifications by phosphorylation and cationization to produce the nanocrystals with the charged surface. Four kinds of SNCs are obtained with the different surface charges involving the varied negative charges, positive charge and no charge. The chemical structures, morphologies and crystalline properties of four SNCs were investigated, together with the effect of surface charges to their cytotoxicity for two cell lines RAW267.4 and CAL27 by the cell proliferation and cell migration assay. The sulfuric acid-hydrolyzed SNC and phosphorylated SNC have more ordered regions and therefore display the higher crystallinities than the enzymatic treated SNC. Four obtained SNCs all exhibited weak cytotoxicity, indicating their good biocompatibility in the potential biomedical application.
本研究以高直链淀粉糯米为原料，通过酸水解和酶处理制备淀粉纳米晶（SNC）。通过磷酸化和阳离子化对制备的 SNC 进行表面改性，得到带有电荷表面的纳米晶。得到了四种具有不同表面电荷的 SNC，分别具有不同的负电荷、正电荷和不带电荷。通过细胞增殖和细胞迁移实验研究了四种 SNC 的化学结构、形态和结晶性能，以及表面电荷对其两种细胞系 RAW267.4 和 CAL27 的细胞毒性的影响。硫酸水解 SNC 和磷酸化 SNC 具有更有序的区域，因此表现出比酶处理 SNC 更高的结晶度。四种获得的 SNC 均表现出较弱的细胞毒性，表明它们在潜在的生物医学应用中具有良好的生物相容性。
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