奇异变形杆菌中质粒DNA在胸腺嘧啶浓度受限情况下的复制
Replication of plasmid DNA in Proteus mirabilis in limiting concentrations of thymine.
作者信息
Womble D D, Warren R L, Rownd R H
出版信息
J Bacteriol. 1978 Mar;133(3):1263-72. doi: 10.1128/jb.133.3.1263-1272.1978.
Abstract
Replicating forms of the R plasmid pRR12 and the colicin E1 plasmid RSF2124 were isolated from Proteus mirabilis after growth in medium containing a limiting concentration of thymine. Both plasmids were replicated as partially supercoiled intermediates, which have densities between the values of covalently closed circular and nicked circular plasmid DNA in ethidium bromide-cesium chloride gradients. In addition, both plasmids had replication intermediates, which have densities lower than that of linear P. mirabilis chromosomal DNA. Some structural features of these replication intermediates were examined.
摘要
在含有极限浓度胸腺嘧啶的培养基中培养后，从奇异变形杆菌中分离出R质粒pRR12和大肠杆菌素E1质粒RSF2124的复制形式。两种质粒均以部分超螺旋中间体的形式复制，在溴化乙锭-氯化铯梯度中，其密度介于共价闭合环状和切口环状质粒DNA的值之间。此外，两种质粒都有复制中间体，其密度低于奇异变形杆菌线性染色体DNA的密度。对这些复制中间体的一些结构特征进行了研究。
相似文献
1
Replication of plasmid DNA in Proteus mirabilis in limiting concentrations of thymine.
J Bacteriol. 1978 Mar;133(3):1263-72. doi: 10.1128/jb.133.3.1263-1272.1978.
2
Effects of inhibition and restoration of protein synthesis on the replication of the R factor Rts1 in Proteus mirabilis.
J Bacteriol. 1974 Feb;117(2):687-95. doi: 10.1128/jb.117.2.687-695.1974.
3
Accumulation of replicating bacterial plasmid DNA during thymine limitation or hydroxyurea treatment.
Mol Gen Genet. 1975 Jul 10;138(4):281-91. doi: 10.1007/BF00264797.
4
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
J Bacteriol. 1975 Jan;121(1):354-62. doi: 10.1128/jb.121.1.354-362.1975.
5
Effect of thymine limitation of chromosomal deoxyribonucleic acid synthesis in Proteus mirabilis.
J Bacteriol. 1972 Sep;111(3):750-7. doi: 10.1128/jb.111.3.750-757.1972.
6
Differential association of F' plasmid and R plasmid deoxyribonucleic acid with a rapidly sedimenting fraction of a Proteus mirabilis lysate.
J Bacteriol. 1977 Jun;130(3):1262-73. doi: 10.1128/jb.130.3.1262-1273.1977.
7
Partially supercoiled replication intermediates of R plasmid DNA are resistant to relaxation.
Nature. 1976 Dec 16;264(5587):676-8. doi: 10.1038/264676a0.
8
Structure and replication of the colicin E1 plasmid.
Curr Top Microbiol Immunol. 1978;83:93-156. doi: 10.1007/978-3-642-67087-9_3.
9
Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.
J Bacteriol. 1977 Apr;130(1):148-53. doi: 10.1128/jb.130.1.148-153.1977.
10
Mode of replication of the conjugative R-plasmid RSF1040 in Escherichia coli.
J Bacteriol. 1976 Apr;126(1):454-66. doi: 10.1128/jb.126.1.454-466.1976.
引用本文的文献
1
Use of thymine limitation and thymine starvation to study bacterial physiology and cytology.
J Bacteriol. 2006 Mar;188(5):1667-79. doi: 10.1128/JB.188.5.1667-1679.2006.
本文引用的文献
1
Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.
Mol Gen Genet. 1975;136(4):273-89. doi: 10.1007/BF00341713.
2
Replication of R-factors in Proteus mirabilis: replication under relaxed control.
J Mol Biol. 1970 Aug;51(3):473-89. doi: 10.1016/0022-2836(70)90002-1.
3
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
4
Asymmetric distribution of guanine plus thymine between complementary strands of deoxyribonucleic acid of members of the Enterobacteriaceae.
J Bacteriol. 1971 Apr;106(1):238-42. doi: 10.1128/jb.106.1.238-242.1971.
5
Studies on extrachromosomal DNA elements. Replication of the colicinogenic factor Col E1 in two temperature sensitive mutants of Escherichia coli defective in DNA replication.
Eur J Biochem. 1970 Aug;15(2):311-20. doi: 10.1111/j.1432-1033.1970.tb01009.x.
6
DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1.
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44. doi: 10.1016/0006-291x(70)90562-0.
7
Pyrimidine metabolism in microorganisms.
Bacteriol Rev. 1970 Sep;34(3):278-343. doi: 10.1128/br.34.3.278-343.1970.
8
Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli.
Nature. 1970 Apr 11;226(5241):126-31. doi: 10.1038/226126a0.
9
Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.
J Biol Chem. 1970 Jan 10;245(1):39-45.
10
Studies on the acid-soluble nucleotide pool in Escherichia coli. IV. Effects of hydroxyurea.
Biochim Biophys Acta. 1967 Aug 22;145(1):1-6. doi: 10.1016/0005-2787(67)90647-8.
Zotero 插件