木质纤维素在稀酸预处理中抗坏血酸辅助分解的核磁共振分析及其对酶解的刺激作用。
Nuclear magnetic resonance analysis of ascorbic acid assisted lignocellulose decomposition in dilute acid pretreatment and its stimulation on enzymatic hydrolysis.
机构信息
Key Laboratory of Forestry Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing 210037, China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
Key Laboratory of Forestry Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing 210037, China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
出版信息
Bioresour Technol. 2022 Jan;343:126147. doi: 10.1016/j.biortech.2021.126147. Epub 2021 Oct 18.
The effect of ascorbic acid addition on lignin decomposition in the dilute acid pretreatment and the corresponding cellulose conversion of pretreated biomass have been studied. This enhancement by ascorbic acid addition was dose dependent. Decomposed and residual bulk lignins from pretreated poplar were analyzed by 2D HSQC and P NMR spectra. The promotional effect on lignocellulose decomposition with the assistance of ascorbic acid addition was supported by the NMR analysis. The analysis showed that the addition of ascorbic acid has a more significant stimulation on decomposed lignins compared to residual bulk lignins. The stimulatory effect of ascorbic acid in lignocellulose decomposition benefits the cellulose conversion of the corresponding pretreated materials. Poplar pretreatment assisted with ascorbic acid (2-8% w/w) increased the final hydrolysis yield by 7.5%-32.2%. This promotional effect of ascorbic acid on enzyme digestibility was more obvious with higher enzyme loading.
研究了抗坏血酸添加对稀酸预处理中木质素分解及其对应预处理生物质中纤维素转化率的影响。这种增强作用与抗坏血酸的添加量呈剂量依赖性。通过二维 HSQC 和 P NMR 光谱分析预处理杨木的分解和残留的整体木质素。NMR 分析支持了抗坏血酸添加在木质纤维素分解中的促进作用。分析表明，与残留的整体木质素相比，添加抗坏血酸对分解木质素的刺激作用更为显著。抗坏血酸在木质纤维素分解中的刺激作用有利于相应预处理材料的纤维素转化率。在杨木预处理中添加抗坏血酸（2-8%w/w）可使最终水解产率提高 7.5%-32.2%。在较高的酶用量下，抗坏血酸对酶解性的促进作用更为明显。
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