Sakalauskaitė Simona, Šaltenienė Violeta, Nikitina Darja, Ugenskienė Rasa, Riškevičienė Vita, Karvelienė Birutė, Juodžiukynienė Nomeda
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Veterinary Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
Laboratory of Clinical and Molecular Gastroenterology, Institute for Digestive Research, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania.
Vet Sci. 2021 Sep 30;8(10):212. doi: 10.3390/vetsci8100212.
Canine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog's breeds has been done. The purpose of this study was to investigate expression of the , , , , , , , , and genes of canine mammary carcinomas, compare the expression levels with clinicopathological parameters and analyze expression disparities between different breeds. Carcinomas and adjacent tissues were collected from female dogs to perform routine histopathology, immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). We found that and genes were overexpressed in the mammary gland carcinomas compared to adjacent tissue. gene expression had associations with different parameters (tumor size, grade, and absence of metastasis). Furthermore, differences in , , , , , and genes expression have been found in different breed dogs (German Shepherd, Yorkshire Terrier) and mixed-breed dogs indicating that a dog's breed could determine a molecular difference, outcome of cancer and should be accounted as a confounding factor in the future gene expression research.
犬乳腺肿瘤（CMTs）是犬类中最常见的癌症之一，也是人类乳腺癌（BC）的良好模型，然而对CMTs的基因表达分析却很匮乏。尽管在不同人种的乳腺癌中已发现基因表达存在差异，但尚未对不同犬种进行此类研究。本研究的目的是调查犬乳腺癌中、、、、、、、和基因的表达情况，将表达水平与临床病理参数进行比较，并分析不同犬种之间的表达差异。从雌性犬收集癌组织和相邻组织，进行常规组织病理学、免疫组织化学（IHC）和定量实时聚合酶链反应（qRT-PCR）。我们发现，与相邻组织相比，和基因在乳腺肿瘤中过表达。基因表达与不同参数（肿瘤大小、分级和无转移）有关。此外，在不同犬种（德国牧羊犬、约克夏梗犬）和混种犬中发现了、、、、和基因表达的差异，这表明犬种可能决定分子差异、癌症结局，在未来的基因表达研究中应将其作为一个混杂因素加以考虑。
