Gu Jianhong, Min Wenyan, Zhao Yutian, Zhang Xueqing, Yuan Yan, Liu Xuezhong, Bian Jianchun, Tong Xishuai, Liu Zongping
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
Vet Sci. 2021 Oct 14;8(10):234. doi: 10.3390/vetsci8100234.
Bones play an important role in maintaining the level of calcium in blood. They provide support for soft tissues and hematopoiesis and undergo continuous renewal throughout life. In addition, vitamin D is involved in regulating bone and calcium homeostasis. Galectin-3 (Gal-3) is a β-galactoside-binding protein that can regulate bone cell differentiation and function. Here, we aimed to study the regulatory effects of Gal-3 on vitamin-D-regulated osteoclastogenesis and bone resorption in chicken. Gal-3 expression in bone marrow stromal cells (BMSCs) from 18-day-old chicken embryos was inhibited or overexpressed. BMSCs were then co-cultured with bone marrow monocytes/macrophages (BMMs) with or without addition of 1α,25(OH)D. The results showed that 1α,25(OH)D upregulated the expression of mRNA and receptor activator of nuclear-factor κB ligand () expression in BMSCs and promoted osteoclastogenesis, as shown by the upregulated expression of osteoclast (OC) markers (, , , and ) and increased bone resorption, a method for measuring the bone resorption area in vitro. Knockdown of by small-interfering RNA (siRNA) in BMSCs downregulated the expression of mRNA and attenuated the effects of 1α,25(OH)D on osteoclastogenesis and bone resorption. Conversely, overexpression of in BMSCs enhanced the effects of osteoclastogenesis and bone resorption by increasing the expression of mRNA. These results demonstrated that Gal-3 mediates the differentiation and bone resorption of osteoclasts regulated by 1α,25(OH)D.
骨骼在维持血液中钙水平方面发挥着重要作用。它们为软组织和造血提供支撑，并在整个生命过程中持续更新。此外，维生素D参与调节骨骼和钙的稳态。半乳糖凝集素-3（Gal-3）是一种β-半乳糖苷结合蛋白，可调节骨细胞的分化和功能。在此，我们旨在研究Gal-3对鸡体内维生素D调节的破骨细胞生成和骨吸收的调控作用。抑制或过表达18日龄鸡胚骨髓基质细胞（BMSC）中Gal-3的表达。然后将BMSC与骨髓单核细胞/巨噬细胞（BMM）共培养，添加或不添加1α,25(OH)D。结果显示，1α,25(OH)D上调了BMSC中mRNA和核因子κB受体激活剂配体（）的表达，并促进了破骨细胞生成，这表现为破骨细胞（OC）标志物（、、、和）表达上调以及骨吸收增加，骨吸收增加是一种体外测量骨吸收面积的方法。通过小干扰RNA（siRNA）敲低BMSC中的可下调mRNA的表达，并减弱1α,25(OH)D对破骨细胞生成和骨吸收的影响。相反，在BMSC中过表达可通过增加mRNA的表达来增强破骨细胞生成和骨吸收的作用。这些结果表明，Gal-3介导了1α,25(OH)D调节的破骨细胞分化和骨吸收。
