College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P. R. China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Poult Sci. 2021 Dec;100(12):101478. doi: 10.1016/j.psj.2021.101478. Epub 2021 Sep 16.
This study aimed to investigate the age-related changes of hepatic metabolism and antioxidant capacity of laying hens at 3 different ages. A total of 192 Hy-line Brown laying hens were assigned into 3 groups: 1) 195-day-old (D195 group); 2) 340-day-old (D340 group); 3) 525-day-old (D525 group). Each group replicated 8 times with 8 hens at the same age. Higher activity of aspartate aminotransferase and lower contents of total protein and globulin were observed in the serum of 525-day-old hens in comparison with their 195-day-old counterparts (P < 0.05). The 525-day-old hens accumulated higher contents of total cholesterol and triglyceride in the liver than 195-day-old birds. Additionally, compared with hens from D195 or D340 group, 525-day-old birds exhibited a lower circulating estradiol level (P < 0.05). For antioxidant capacity, birds in the D525 group showed a higher malondialdehyde concentration in both serum and liver as compared with D195 or D340 group (P < 0.05). The 525-day-old hens also exhibited lower glutathione peroxidase activities in both serum and liver when compared with 195-day-old birds (P < 0.05). Simultaneously, there was a decline of hepatic superoxide dismutase activity in the D525 group in comparison with D195 group (P < 0.05). Compared with 195-day-old counterparts, 340-day-old birds upregulated the mRNA abundance of nuclear factor erythroid-2 related factor 2 and glutathione peroxidase 1 in the liver (P < 0.05). In contrast, hens from D525 group showed the downregulation of hepatic nuclear factor erythroid-2 related factor 2, NAD(P)H quinone dehydrogenase 1, and superoxide dismutase 1 when compared with D340 group (P < 0.05). These results indicated that increasing age can adversely affect liver metabolism and function of laying hens.
本研究旨在探讨 3 个不同日龄产蛋鸡的肝脏代谢和抗氧化能力的年龄相关变化。将 192 只海兰褐产蛋鸡分为 3 组：1）195 日龄组（D195 组）；2）340 日龄组（D340 组）；3）525 日龄组（D525 组）。每组重复 8 次，每次 8 只相同日龄的鸡。与 195 日龄鸡相比，525 日龄鸡血清中天冬氨酸转氨酶活性更高，总蛋白和球蛋白含量更低（P < 0.05）。525 日龄鸡肝脏总胆固醇和甘油三酯含量高于 195 日龄鸡。此外，与 D195 或 D340 组相比，525 日龄鸡的循环雌二醇水平较低（P < 0.05）。就抗氧化能力而言，与 D195 或 D340 组相比，D525 组鸡血清和肝脏中的丙二醛浓度均较高（P < 0.05）。与 195 日龄鸡相比，525 日龄鸡血清和肝脏中的谷胱甘肽过氧化物酶活性较低（P < 0.05）。同时，与 D195 组相比，D525 组鸡肝脏中超氧化物歧化酶活性下降（P < 0.05）。与 195 日龄鸡相比，340 日龄鸡肝脏中核因子红细胞 2 相关因子 2 和谷胱甘肽过氧化物酶 1 的 mRNA 丰度上调（P < 0.05）。相比之下，与 D340 组相比，D525 组鸡肝脏中核因子红细胞 2 相关因子 2、NAD（P）H 醌氧化还原酶 1 和超氧化物歧化酶 1 的表达下调（P < 0.05）。这些结果表明，随着年龄的增长，会对产蛋鸡的肝脏代谢和功能产生不利影响。
