Hoosein N M, Brattain D E, McKnight M K, Levine A E, Brattain M G
Cancer Res. 1987 Jun 1;47(11):2950-4.
The effects of transforming growth factor-beta (TGF beta) on a human colon carcinoma cell line (MOSER) were investigated. TGF beta, at low concentrations (between 0.1 and 1.0 ng/ml), inhibited the proliferation of MOSER cells both in monolayer culture and soft agarose, in a dose-dependent manner. MOSER cells adapted to growth in chemically defined serum-free medium were more sensitive to the inhibitory effects of TGF beta than cells maintained in serum-supplemented medium. Morphological changes in MOSER cells, observed with TGF beta, were similar to those seen with the chemical differentiation agent N,N-dimethylformamide. Also in similarity to the effects of N,N-dimethylformamide, TGF beta induced a time- and concentration-dependent increase in soluble extracellular fibronectin. Binding studies with [125I]TGF beta revealed a relatively low number of binding sites on MOSER cells (13%) compared with mouse embryo fibroblastic (AKR-2B) cells. Thus far, other colon carcinoma cell lines, some displaying TGF beta receptors, have been reported to be unresponsive to TGF beta. This study is therefore the first to demonstrate a TGF beta-responsive colon carcinoma cell line.
研究了转化生长因子-β（TGF-β）对人结肠癌细胞系（MOSER）的影响。低浓度（0.1至1.0 ng/ml）的TGF-β以剂量依赖方式抑制MOSER细胞在单层培养和软琼脂糖中的增殖。适应在化学成分明确的无血清培养基中生长的MOSER细胞比在补充血清的培养基中培养的细胞对TGF-β的抑制作用更敏感。用TGF-β观察到的MOSER细胞形态变化与用化学分化剂N，N-二甲基甲酰胺观察到的相似。同样与N，N-二甲基甲酰胺的作用相似，TGF-β诱导可溶性细胞外纤连蛋白随时间和浓度依赖性增加。与[125I]TGF-β的结合研究显示，与小鼠胚胎成纤维细胞（AKR-2B）相比，MOSER细胞上的结合位点数量相对较少（13%）。迄今为止，已有报道称其他一些显示TGF-β受体的结肠癌细胞系对TGF-β无反应。因此，本研究首次证明了一种对TGF-β有反应的结肠癌细胞系。
