Specific two-photon fluorescent probe for cysteine detection in vivo.

Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases. Herein, a new fluorescent probe, ANP, which is based on 6-acetyl-N-methyl-2-naphthyl amine, has been developed for Cys detection over Hcy and GSH in vivo. The addition of thiol on α,β-unsaturated ketone promotes 87-fold fluorescence turn-on response with a 65 nM limit of detection. The high two-photon efficiency of the probe ANP (cross-section = 22.3) makes it a suitable probe for evaluating Cys in living cells without background fluorescence interference. Its application was extended to monitor the Cys distribution in live cells and tissues.