Xing Dawei, Li Tongtong, Ma Guoliang, Ruan Haixiang, Gao Liping, Xia Tao
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
School of Life Sciences, Anhui Agricultural University, Hefei, China.
Front Plant Sci. 2021 Oct 21;12:733287. doi: 10.3389/fpls.2021.733287. eCollection 2021.
The ubiquitin/26S proteasome pathway is a critical protein-degradation pathway in plant growth and development as well as in nearly all biological and abiotic stress processes. Although as a member of the ubiquitin/26S proteasome pathway, the E3 ubiquitin ligase family has been shown to be essential for the selective degradation of downstream target proteins, it has been rarely reported in tea plants (). In this study, through database searches and extensive manual deduplication, 335 RING finger family proteins were selected from the Tea Plant Information Archive. These proteins were divided into six categories by the difference of RING finger domain: RING-H2, RING-HCa, RING-HCb, RING-C2, RING-v, and RING-G. Stress-induced differential gene expression analysis showed that 53 proteins in RING finger family can respond to selected exogenous stress. ubiquitination assays indicated that TEA031033, which was named CsMIEL1, exhibited the activity of E3 ubiquitin ligases. transgenic seedlings were resistant to some exogenous abiotic stresses, such as salt and drought stress but sensitive to exogenous methyl jasmonate treatment. Furthermore, CsMIEL1 reduced the accumulation of anthocyanin in transgenic plants in response to low temperature treatment. The results of this article provide basic date for studying the role of ubiquitin/26S proteasome pathway in tea plants response to stresses.
泛素/26S蛋白酶体途径是植物生长发育以及几乎所有生物和非生物胁迫过程中的关键蛋白质降解途径。尽管作为泛素/26S蛋白酶体途径的成员，E3泛素连接酶家族已被证明对下游靶蛋白的选择性降解至关重要，但在茶树中却鲜有报道（）。在本研究中，通过数据库搜索和广泛的手动去重，从茶树信息档案库中筛选出335个RING指蛋白家族蛋白。这些蛋白根据RING指结构域的差异分为六类：RING-H2、RING-HCa、RING-HCb、RING-C2、RING-v和RING-G。胁迫诱导的差异基因表达分析表明，RING指蛋白家族中的53种蛋白能够对选定的外源胁迫作出反应。泛素化分析表明，命名为CsMIEL1的TEA031033具有E3泛素连接酶活性。转基因幼苗对一些外源非生物胁迫具有抗性，如盐胁迫和干旱胁迫，但对外源茉莉酸甲酯处理敏感。此外，CsMIEL1在低温处理下降低了转基因植物中花青素的积累。本文的研究结果为研究泛素/26S蛋白酶体途径在茶树应对胁迫中的作用提供了基础数据。
