College of Tropic Crops, Hainan University, Haikou, China.
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou, China.
Plant Signal Behav. 2021 Dec 2;16(12):1995647. doi: 10.1080/15592324.2021.1995647. Epub 2021 Nov 9.
Iron (Fe) and Zinc (Zn) are essential nutrient elements for plant growth and development. Here, we observed the effects of Fe and Zn deficiency in seedlings of L. (areca palm), one of the most cultured palm trees in tropic regions. Results revealed that Fe deficiency causes strong chlorosis with the significantly decreased chlorophyll biosynthesis level and photosynthetic activities in the top third young leaf (L3) of seedlings. Zn deficiency caused light chlorosis in all three young leaves which slightly decreased chlorophyll biosynthesis and photosynthetic activities. Analysis of the Fe and Zn concentration in leaves and roots indicated that absorption and distribution of these two ions share cooperative pathways, since Zn deficiency caused Fe increasing, and vice versa. Therefore, we focused on the genes in areca trees. From the whole-genome data set we obtained, 6 genes were classified, and a phylogenetic tree was constructed with other 38 genes from model plants to find their potential functions. We also analyzed the expression pattern of genes under Zn and Fe deficiency by transcriptomic approaches. With these results, we constructed an expression atlas of genes in leaves and roots of areca seedlings with the dynamic expression levels under Fe and Zn deficient conditions. In conclusion, we provide evidence to understand the absorption and transport of nutrient elements, Fe and Zn, in the tropic agricultural plant
铁（Fe）和锌（Zn）是植物生长和发育所必需的营养元素。在这里，我们观察到铁和锌缺乏对热带地区最常见的棕榈树之一 L.（槟榔树）幼苗的影响。结果表明，铁缺乏导致强烈的黄化，导致顶部第三片幼叶（L3）中的叶绿素生物合成水平和光合作用活性显著降低。锌缺乏导致所有三片幼叶出现轻度黄化，略微降低叶绿素生物合成和光合作用活性。叶片和根系中 Fe 和 Zn 浓度的分析表明，这两种离子的吸收和分布共享合作途径，因为锌缺乏导致 Fe 增加，反之亦然。因此，我们专注于槟榔树的基因。从我们获得的全基因组数据集，我们将 6 个基因分类，并与来自模式植物的其他 38 个基因构建了一个系统发育树，以寻找它们的潜在功能。我们还通过转录组学方法分析了基因在 Zn 和 Fe 缺乏下的表达模式。有了这些结果，我们构建了一个槟榔幼苗叶片和根系中基因的表达图谱，显示了在 Fe 和 Zn 缺乏条件下的动态表达水平。总之，我们提供了证据来理解热带农业植物中营养元素 Fe 和 Zn 的吸收和运输。
