Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, P. R. China.
MOE Key laboratory of Laser Life Science & Institute of Laser Life Science College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. China.
Analyst. 2021 Nov 22;146(23):7320-7326. doi: 10.1039/d1an01705e.
A new and simple surface-enhanced Raman scattering (SERS) biosensor based on the tyramine signal amplification (TSA)-triggered formation of horseradish peroxidase (HRP) repeats on a gold sensing chip was designed for the highly sensitive detection of hydrogen peroxide (HO). Initially, gold wafers were functionalized with HRP as sensing chips. Then, the HRP immobilized on the chips triggers the TSA reaction to transform the tyramine-HRP conjugate into a tyramine-HRP repeat array. With the aid of the target HO, the HRP repeats catalyze the oxidation of -phenylenediamine (OPD) and produce an enzyme catalytic product with a different chemical structure, thus altering the fingerprint of the SERS spectra from that of OPD. HO can be quantitatively analyzed according to the change in SERS signal intensity. On the basis of the TSA strategy, the proposed method allows the detection of HO with a limit of detection (LOD) of 0.8 × 10 M. The as-prepared SERS sensor can achieve high-sensitivity HO detection with a small amount of sample for each analysis. Therefore, this sensor exhibits significant potential for application in bioanalysis.
一种新的基于辣根过氧化物酶（HRP）重复在金传感芯片上的酪胺信号放大（TSA）触发形成的简单表面增强拉曼散射（SERS）生物传感器，被设计用于对过氧化氢（HO）的高灵敏度检测。最初，金片用 HRP 功能化作为传感芯片。然后，固定在芯片上的 HRP 触发 TSA 反应，将酪胺-HRP 缀合物转化为酪胺-HRP 重复阵列。在目标 HO 的辅助下，HRP 重复催化 -苯二胺（OPD）的氧化，并产生具有不同化学结构的酶催化产物，从而改变 SERS 光谱的指纹图谱与 OPD 的指纹图谱不同。根据 SERS 信号强度的变化可以定量分析 HO。基于 TSA 策略，所提出的方法允许以 0.8×10^-7 M 的检测限（LOD）检测 HO。所制备的 SERS 传感器可以实现高灵敏度的 HO 检测，每次分析只需少量样品。因此，这种传感器在生物分析中具有重要的应用潜力。
