甲状腺功能生物标志物在24小时内的生物学变异

Biological variation of thyroid function biomarkers over 24 hours.

作者信息

Zhang Yan, He Da-Hai, Jiang Shun-Ning, Wang Hua-Li, Xu Xiao-Hua, Kong Li-Rui

机构信息

Department of Clinical Laboratory, Traditional Chinese Medicine Hospital of Pidu District, Chengdu, China.

出版信息

Clin Chim Acta. 2021 Dec;523:519-524. doi: 10.1016/j.cca.2021.11.007. Epub 2021 Nov 8.

DOI:10.1016/j.cca.2021.11.007

PMID:34762936

Abstract

BACKGROUND

Thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), free T3 (FT3), and free T4 (FT4) are used to diagnose thyroid diseases and monitor treatment effects. Reliable biological variation (BV) data is required to ensure accurate clinical decisions.

METHODS

Blood samples were collected from 31 healthy subjects at 00:00, 04:00, 08:00, 12:00, 16:00, and 20:00; each sample was analyzed twice for TSH, T3, T4, FT3, and FT4. After outlier exclusion, normality assessment, and variance homogeneity, sex-stratified BV, including within-subject (CV) and between-subject (CV), was defined using nested ANOVA.

RESULTS

Concentrations of five biomarkers were significantly different between sexes. The CV and CV estimates were 34.54% and 34.43% for TSH, 5.89% and 14.18% for T3, 4.48% and 14.96% for T4, 5.37% and 11.23% for FT3, and 3.57% and 8.03% for FT4, respectively. The individual indexes (IIs) of all the biomarkers (except TSH) were ≤ 0.63. Males had lower CVs and IIs than females.

CONCLUSION

CV estimates of all hormones, except TSH, were lower than those reported on the BV website, showing low IIs and differences between sexes. We provide updated data on the short-term BV of thyroid function biomarkers according to sex and complement BV data of thyroid function biomarkers.

摘要

背景

促甲状腺激素（TSH）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、游离T3（FT3）和游离T4（FT4）用于诊断甲状腺疾病并监测治疗效果。需要可靠的生物学变异（BV）数据以确保准确的临床决策。

方法

在00:00、04:00、08:00、12:00、16:00和20:00从31名健康受试者采集血样；每个样本对TSH、T3、T4、FT3和FT4进行两次分析。在排除异常值、评估正态性和方差齐性后，使用嵌套方差分析定义按性别分层的BV，包括个体内变异（CV）和个体间变异（CV）。

结果

五种生物标志物的浓度在性别之间存在显著差异。TSH的CV和CV估计值分别为34.54%和34.43%，T3为5.89%和14.18%，T4为4.48%和14.96%，FT3为5.37%和11.23%，FT4为3.57%和8.03%。所有生物标志物（TSH除外）的个体指数（IIs）均≤0.63。男性的CV和IIs低于女性。