Key Laboratory of Hydraulic and Waterway Engineering of the Ministry of Education, School of River and Ocean Engineering, Chongqing Jiaotong University, Chongqing 400074, PR China; Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China; Research Center for Pollution Control and Ecological Restoration, Yuxi Normal University, Yuxi 653100, Yunnan, PR China.
Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China.
Bioresour Technol. 2022 Mar;347:126339. doi: 10.1016/j.biortech.2021.126339. Epub 2021 Nov 11.
Microbial biomass and activity are frequently subjected to organic loading shock (OLS) from decentralized municipal wastewater. A hybrid moving bed biofilm reactor-assisted dual-anaerobic-anoxic/oxic system (D-AMBBR) was established by integrating dual-anaerobic-anoxic/oxic with moving bed biofilm reactor to resist OLS for stable nutrients removal. The D-AMBBR achieved 91.57% of chemical oxygen demand, 93.33% of ammonia-nitrogen, 80.20% of total nitrogen and 92.68% of total phosphorus removal, respectively, under the fluctuation of organic loading rate from 417.9 to 812.0 g COD m d. The 16S rRNA gene sequencing revealed that Gemmobacter (7.28%) was identified as dominating anoxic denitrifying genus in oxic chamber, confirming the coexistence of aerobic and anaerobic/anoxic micro-environments. This circumstance boosted simultaneous nitrification-denitrification and phosphorus removal and the microbial community evolution inside the multilayer biocarrier-attached biofilms. In general, the D-AMBBR was able to provide unique, cooperative and robust bacterial consortia to form a buffer against OLS, and ensuring effluent stability.
微生物生物量和活性经常受到来自分散式城市污水的有机负荷冲击（OLS）的影响。通过将双厌氧-缺氧/好氧与移动床生物膜反应器相结合，建立了一种混合移动床生物膜反应器辅助双厌氧-缺氧/好氧系统（D-AMBBR），以抵抗 OLS 以实现稳定的养分去除。在有机负荷率从 417.9 到 812.0 g COD m d 的波动下，D-AMBBR 分别实现了 91.57%的化学需氧量、93.33%的氨氮、80.20%的总氮和 92.68%的总磷去除。16S rRNA 基因测序显示，好氧室中优势缺氧反硝化属为 Gemmobacter（7.28%），证实了好氧和厌氧/缺氧微环境的共存。这种情况促进了同步硝化-反硝化和磷去除以及多层生物载体附着生物膜内的微生物群落演化。总的来说，D-AMBBR 能够提供独特、合作和强大的细菌群落，形成对 OLS 的缓冲，确保出水稳定。
