Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, P.O. Box 115, Shahrekord, Iran.
Institute of Biotechnology, Shahrekord University, P.O. Box 115, Shahrekord, Iran.
Mol Biol Rep. 2022 Feb;49(2):1121-1132. doi: 10.1007/s11033-021-06938-1. Epub 2021 Nov 15.
Novel strategies for improvement of ornamental plants and their properties relay on miRNA control of differential plant gene expression modulation. Still, in response to the same abiotic stresses, some conserved miRNA families show different expression patterns in different plant species. In parallel, the use of deep sequencing technologies reveals new levels of complexity of regulatory networks in plants through identification of new miRNAs.
Fritillaria imperialis plants were collected from their natural habitats in Koohrang, Chaharmahal va Bakhtiari, Iran. Several tissues including stamen, pistil, petal, sepal, leaf, stem, bulb and fruit were collected during three developmental stages (stem elongation, flower development and seed head stages). Using RNAseq and qRT-PCR approach, this research revealed 21 conserved miRNAs, matching 15 miRNA families, in Fritilaria imperialis.
The expression of seven conserved miRNAs (Fim-miR156b, Fim-miR159, Fim-miR166a-5p, Fim-miR169d-5p, Fim-miR171c, Fim-miR393 and Fim-miR396e-3p) was further investigated in different tissues and three developmental stages, suggesting different roles for these miRNAs during growth and development of crown imperial. Gained knowledge from this research can open the door to find efficient ways to secure crown imperial survival, preservation and utilization and if proven useful may be applied in other plant species as well.
改良观赏植物及其特性的新策略依赖于 miRNA 对植物基因表达差异调控的控制。然而，对于相同的非生物胁迫，一些保守的 miRNA 家族在不同的植物物种中表现出不同的表达模式。与此同时，通过鉴定新的 miRNA，深度测序技术的使用揭示了植物调控网络的新层次的复杂性。
从伊朗科赫朗的自然栖息地收集了贝母属植物。在三个发育阶段（茎伸长、花发育和种子头阶段）收集了包括雄蕊、雌蕊、花瓣、萼片、叶、茎、鳞茎和果实在内的几种组织。通过 RNAseq 和 qRT-PCR 方法，本研究在 Fritilaria imperialis 中发现了 21 个保守的 miRNA，匹配 15 个 miRNA 家族。
在不同组织和三个发育阶段进一步研究了七个保守 miRNA（Fim-miR156b、Fim-miR159、Fim-miR166a-5p、Fim-miR169d-5p、Fim-miR171c、Fim-miR393 和 Fim-miR396e-3p）的表达情况，表明这些 miRNA 在皇冠贝母的生长和发育过程中具有不同的作用。从这项研究中获得的知识可以为寻找有效保护皇冠贝母生存、保存和利用的方法打开大门，如果证明有用，也可以应用于其他植物物种。
