Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India; CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 XinGang Road West, Guangzhou 510301, China.
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India.
J Infect Public Health. 2021 Dec;14(12):1831-1841. doi: 10.1016/j.jiph.2021.11.001. Epub 2021 Nov 9.
Actinomycetes particularly, Streptomyces species are producing wide variety of natural products with potential bioactivities. The microbial-derived metabolites hold a strong position to combat emerging and re-emerging antimicrobial drug-resistant pathogens.
A diverse group of actinomycetes strains were isolated from unexplored regions of mangrove sediment. Further, a polyphasic approach based on 16S rRNA gene sequence analysis and to evaluate their antibacterial potential against a panel of bacterial pathogens and methicillin resistance Staphylococcus aureus (MRSA).
The mangrove sediment samples were serially diluted with sterile water and plated on inorganic starch agar medium. A total of 20 isolates were pure cultured and 16S rRNA gene sequences were deposited in the public nucleotide databases (GenBank, NCBI). All the isolates were screened for the antibacterial activity by agar overlay method. Further, the susceptibility pattern of MRSA by flow cytometry and fluorescence microscopy was analysed.
These twenty different isolates were grouped under nine major clad and they shared 95-99% sequence identity to the 16S rRNA gene sequences of the genus Streptomyces in the public nucleotide databases. Among these strains, the isolates namely JRG-02, JRG-03, JRG-04, JRG-10 and JRG-12 exhibited a broad-spectrum antibacterial activity against Methicillin-resistant Staphylococcus aureus(MRSA) and Gram negative bacteria Klebsiella pneumoniae MTCC109. Furthermore, we have characterized the antibacterial compound production and its properties from the isolate JRG-02, a potential drug candidate. The culture conditions and various nutrient components of strain Streptomyces sp. JRG-02 were optimized for enhanced antibiotics production of the isolate. The FT-IR and LCMS spectrum analysis envisaged the chemical nature of the substance. The effect of antibacterial compound on the viability of MRSA was alone examined by flow cytometry (FACS) and fluorescence microscopy analysis.
The present study clearly shows that the survival of diverse inhabitants of Streptomyces in the mangrove sediments. Hence, the mangrove sediment inhabiting strain Streptomyces sp. JRG-02 has potential pharmaceutical activity and genetic diversity.
放线菌，尤其是链霉菌属，能够产生具有潜在生物活性的多种天然产物。微生物来源的代谢产物在对抗新出现和重新出现的抗微生物药物耐药病原体方面具有强大的地位。
从红树林沉积物的未探索区域分离出一组多样化的放线菌菌株。进一步，基于 16S rRNA 基因序列分析和评估它们对一组细菌病原体和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌潜力的多相方法。
用无菌水对红树林沉积物样品进行连续稀释，然后接种在无机淀粉琼脂培养基上。总共分离出 20 株纯培养物，并将 16S rRNA 基因序列提交到公共核苷酸数据库（GenBank，NCBI）。通过琼脂覆盖法筛选所有分离物的抗菌活性。进一步，通过流式细胞术和荧光显微镜分析 MRSA 的药敏模式。
这 20 个不同的分离物分为 9 个主要群，它们与公共核苷酸数据库中链霉菌属的 16S rRNA 基因序列的相似度为 95-99%。在这些菌株中，分离株 JRG-02、JRG-03、JRG-04、JRG-10 和 JRG-12 对耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性菌肺炎克雷伯菌 MTCC109 表现出广谱抗菌活性。此外，我们从分离株 JRG-02 中鉴定出一种潜在的候选药物的抗菌化合物的产生及其特性。优化了菌株链霉菌 sp. JRG-02 的培养条件和各种营养成分，以提高其抗生素的产生。FT-IR 和 LCMS 光谱分析预测了物质的化学性质。通过流式细胞术（FACS）和荧光显微镜分析单独检查了抗菌化合物对 MRSA 活力的影响。
本研究清楚地表明，红树林沉积物中存在多样化的链霉菌居民的生存。因此，红树林沉积物栖息的链霉菌 sp. JRG-02 具有潜在的药物活性和遗传多样性。
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