Fujihara Rina, Uchida Naoyuki, Tameshige Toshiaki, Kawamoto Nozomi, Hotokezaka Yugo, Higaki Takumi, Simon Rüdiger, Torii Keiko U, Tasaka Masao, Aida Mitsuhiro
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Center for Gene Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.
Plant Biotechnol (Tokyo). 2021 Sep 25;38(3):317-322. doi: 10.5511/plantbiotechnology.21.0508a.
The shoot organ boundaries have important roles in plant growth and morphogenesis. It has been reported that a gene encoding a cysteine-rich secreted peptide of the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family, , is expressed in the boundary domain between the two cotyledon primordia of embryo. However, its developmental functions remain unknown. This study aimed to analyze the role of during embryogenesis. We found that cotyledon growth was reduced in its loss-of-function mutants, and this phenotype was associated with the reduction of auxin response peaks at the tips of the primordia. The reduced cotyledon size of the mutant embryo recovered in germinating seedlings, indicating the presence of a factor that acted redundantly with to promote cotyledon growth in late embryogenesis. Our analysis suggests that the boundary domain between the cotyledon primordia acts as a signaling center that organizes auxin response peaks and promotes cotyledon growth.
茎器官边界在植物生长和形态发生中具有重要作用。据报道，一个编码表皮模式因子样（EPFL）家族富含半胱氨酸分泌肽的基因，在胚胎的两个子叶原基之间的边界区域表达。然而，其发育功能仍不清楚。本研究旨在分析该基因在胚胎发生过程中的作用。我们发现，其功能缺失突变体中子叶生长受到抑制，且该表型与原基顶端生长素反应峰值的降低有关。突变体胚胎子叶大小减小的情况在萌发的幼苗中得以恢复，这表明存在一个与该基因冗余作用的因子，在胚胎发育后期促进子叶生长。我们的分析表明，子叶原基之间的边界区域作为一个信号中心，组织生长素反应峰值并促进子叶生长。
