Departamento de microbiologia e parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Faculdade de medicina veterinária, Universidade Estácio de Sá, Rio de Janeiro, Brazil.
Lett Appl Microbiol. 2022 Feb;74(2):124-130. doi: 10.1111/lam.13601. Epub 2021 Nov 24.
The culturing of Leptospira strains from bovine clinical samples is challenging and has resulted in some gaps in securing an epidemiological understanding. Strains related to chronic reproductive leptospirosis in cattle belong to the Sejroe serogroup - not only Hardjoprajitno and Hardjobovis but also Guaricura genotypes. This study analyses the growth of Leptospira strains from serogroup Sejroe in different culture media, with the aim of suggesting better culturing approaches. To meet this objective, two culture media were applied: EMJH and T80/40/LH. In addition, three different cocktails of selective agents were chosen. The combinations of medium and selective additives resulted in 10 different tested formulae. The poor performance of Hardjobovis in EMJH indicated that its growth may represent a possible bias when culturing these strains from bovine samples. The most efficient medium for culturing Hardjobovis was T80/40/LH, while T80/40/LH medium + STAFF combination proved to be the best choice for growth, being recommended for obtaining a higher number of these strains from bovines.
从牛临床样本中培养钩端螺旋体菌株具有挑战性,这导致在确保流行病学理解方面存在一些差距。与牛慢性生殖性钩端螺旋体病相关的菌株属于塞罗血清群——不仅有哈日株和波摩那株,还有瓜里夸拉基因型。本研究分析了不同培养基中塞罗血清群钩端螺旋体菌株的生长情况,旨在提出更好的培养方法。为了实现这一目标,应用了两种培养基:EMJH 和 T80/40/LH。此外,还选择了三种不同的选择性试剂鸡尾酒。培养基和选择性添加剂的组合产生了 10 种不同的测试配方。哈日株在 EMJH 中的表现不佳表明,在从牛样本中培养这些菌株时,其生长可能代表了一种可能的偏差。最适合培养哈日株的培养基是 T80/40/LH,而 T80/40/LH 培养基+STAFF 组合被证明是最佳的生长选择,建议用于从牛中获得更多的这些菌株。