National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
J Vet Med Sci. 2022 Jan 7;84(1):1-5. doi: 10.1292/jvms.21-0490. Epub 2021 Nov 17.
Recently, genotype VII of Newcastle disease virus (NDV) has become the most prevalent NDV genotype in Asia. Here the hemagglutinin-neuraminidase (HN) gene of genotype VII NDV strains isolated in Japan was analyzed. Notably, point amino acid substitutions in the HN protein at position 347, which is located on the major linear epitope of the HN protein, were found in two strains. However, by a hemagglutination inhibition assay, major antigenic differences did not exist between the studied strains. Additionally, chickens vaccinated with the B1 strain did not exhibit clinical effects after challenge with variants possessing the substitution at position 347 (E to K), whereas all unvaccinated chickens subjected to this challenge died within 5 days.
最近，新城疫病毒（NDV）的基因型 VII 已成为亚洲最流行的 NDV 基因型。本研究对日本分离的 VII 型 NDV 株的血凝素-神经氨酸酶（HN）基因进行了分析。值得注意的是，在位于 HN 蛋白主要线性表位的 347 位氨基酸发生了点突变，这两个分离株都存在。然而，通过血凝抑制试验，研究的分离株之间并没有明显的抗原差异。此外，用 B1 株免疫的鸡在攻毒时，在位置 347 （E 到 K）上发生替换的变异株不会出现临床效果，而所有未接种的鸡在攻毒后 5 天内全部死亡。
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