Jiang Haiyan, Dong Yansong, Yan Dajun, Wu Yao, Wang Yue, Ren Yuting, Mao Guomin, Liang Guiwen, Liu Wei, Zhou Yang, Huang Zhongwei, Qi Lei
Department of Health Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Department of Emergency Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Ann Transl Med. 2021 Oct;9(20):1519. doi: 10.21037/atm-21-2794.
Sepsis is a systemic disease characterized by extensive inflammatory responses and impaired organ function, which are characteristics that make it easily missed and complex to treat. A large number of laboratory and clinical studies on the diagnosis and treatment of sepsis have been continuously carried out, confirming the importance of mitochondrial function during the development of sepsis. STEAP4 is an important metalloreductase in mitochondria, which is involved in the biogenesis and respiratory chain of mitochondria. The role of STEAP4 in inflammation remains controversial. Research in this field may contribute to the development of new diagnostic and treatment options for sepsis.
The expression of STEAP4 was measured in the peripheral blood of patients with severe sepsis and compared with healthy controls. Cell and mouse inflammatory models were established to detect the expression of STEAP4 and other inflammatory cytokines.
(I) The expression of STEAP4 in the peripheral blood of patients with severe sepsis is higher than that of healthy volunteers (P<0.01), which is related to the SOFA score and transaminase. (II) STEAP4 has a certain predictive effect on the outcome of patients [area under curve (AUC) =0.696, P<0.05, 95% CI: 0.528 to 0.833]. (III) Inflammation led to increased expression of gene in RAW264.7 cells and mouse liver tissue.
The expression of STEAP4 is elevated in the early stage of sepsis and the degree of its elevation can be used to predict the clinical outcome of sepsis patients.
脓毒症是一种以广泛炎症反应和器官功能受损为特征的全身性疾病，这些特征使其容易被漏诊且治疗复杂。关于脓毒症诊断和治疗的大量实验室及临床研究一直在持续进行，证实了线粒体功能在脓毒症发生发展过程中的重要性。STEAP4是线粒体中的一种重要金属还原酶，参与线粒体的生物发生和呼吸链。STEAP4在炎症中的作用仍存在争议。该领域的研究可能有助于开发脓毒症新的诊断和治疗方案。
检测重症脓毒症患者外周血中STEAP4的表达，并与健康对照进行比较。建立细胞和小鼠炎症模型，以检测STEAP4及其他炎症细胞因子的表达。
（I）重症脓毒症患者外周血中STEAP4的表达高于健康志愿者（P<0.01），且与序贯器官衰竭评估（SOFA）评分及转氨酶相关。（II）STEAP4对患者预后有一定预测作用[曲线下面积（AUC）=0.696，P<0.05，95%可信区间：0.528至0.833]。（III）炎症导致RAW264.7细胞和小鼠肝脏组织中该基因表达增加。
脓毒症早期STEAP4表达升高，其升高程度可用于预测脓毒症患者的临床结局。
