Min Wenjiao, Tang Nie, Zou Zhili, Chen Yuexin, Zhang Xu, Huang Yulan, Wang Jinyu, Zhang Yaoyin, Zhou Bo, Sun Xueli
Psychosomatic Department, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China.
Mental Health Center, West China University Hospital, Sichuan University, Chengdu, China.
Ann Transl Med. 2021 Oct;9(20):1555. doi: 10.21037/atm-21-4803.
Biological rhythm is closely related to health. We aimed to identify the potential correlations of rhythm gene polymorphisms to type 2 diabetes mellitus (DM) or bipolar disorder (BD), which both have many abnormal rhythmic activities, in a sample of Chinese Han origin.
A total of 136 patients with BD, 166 patients with DM, and 130 healthy controls were collected. We screened 28 single nucleotide polymorphisms (SNPs) located in rhythm genes CLOCK, ARNTL, PER2, PER3, CRY1, and CRY2 respectively. Snapshot typing technology was used for genotyping.
Both the rs10832022-G and rs11022765-A allele frequencies of the ARNTL gene were significantly higher in patients with DM than in those with BD (corrected P=0.03, 0.004, respectively). The frequency of rs10832022-G, rs1022765-A, and rs11022762-T haplotypes, which was significantly lower in patients with BD than in controls (P=0.003, OR =0.579), was significantly higher in patients with DM than in those with BD (P=0.0002, OR =1.878). The rs2292910-CC genotypic frequency of the CRY2 gene was significantly higher in patients with BD than in controls (OR of regression =2.203, P=0.01), while the frequency of the AA genotype was significantly lower than in patients with DM (P=0.01). The frequency of rs1972874-G and rs36124720-G haplotype of the PER2 gene was significantly higher in patients with DM than in controls (P=0.01, OR =1.577).
Our study preliminarily suggested that both BD and type 2 DM could be considered as dysrhythmias with different rhythmic genetic backgrounds, which contribute to the early prediction of an individual's susceptibility to different rhythm disorders and early intervention.
生物节律与健康密切相关。我们旨在在中国汉族样本中确定节律基因多态性与2型糖尿病（DM）或双相情感障碍（BD）之间的潜在相关性，这两种疾病都有许多异常的节律活动。
共收集了136例BD患者、166例DM患者和130名健康对照者。我们分别筛选了位于节律基因CLOCK、ARNTL、PER2、PER3、CRY1和CRY2中的28个单核苷酸多态性（SNP）。采用Snapshot分型技术进行基因分型。
ARNTL基因的rs10832022 - G和rs11022765 - A等位基因频率在DM患者中显著高于BD患者（校正P值分别为0.03和0.004）。rs10832022 - G、rs1022765 - A和rs11022762 - T单倍型频率在BD患者中显著低于对照者（P = 0.003，OR = 0.579），而在DM患者中显著高于BD患者（P = 0.0002，OR = 1.878）。CRY2基因的rs2292910 - CC基因型频率在BD患者中显著高于对照者（回归OR = 2.203，P = 0.01），而AA基因型频率显著低于DM患者（P = 0.01）。PER2基因的rs1972874 - G和rs36124720 - G单倍型频率在DM患者中显著高于对照者（P = 0.01，OR = 1.577）。
我们的研究初步表明，BD和2型DM都可被视为具有不同节律遗传背景的节律紊乱，这有助于早期预测个体对不同节律障碍的易感性并进行早期干预。
