Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.
Oxid Med Cell Longev. 2021 Nov 8;2021:6485871. doi: 10.1155/2021/6485871. eCollection 2021.
Hepatocellular carcinoma (HCC) is an aggressive malignant tumor with a poor prognosis. Reactive oxygen species (ROS) play an important role in tumors; however, the role of ROS-related genes is still unclear in HCC. Therefore, we analyzed the role of ROS-related genes in HCC via bioinformatics methods. Firstly, a prognosis model was constructed using LASSO Cox regression and multivariate analyses. We also investigated the potential function of the ROS-related genes and the correlation with immune infiltration, tumor stemness, and drug sensitivity. ICGC database was used for validation. Secondly, we further analyzed the role of 11 ROS-related genes in HCC. As a member of ROS gene family, the role of STK25 has remained unclear in HCC. We explored the biological function of STK25 using experiments. The present study was the first to construct a ROS-related prognostic model in HCC. The correlation of ROS-related genes with immune infiltration, tumor stemness, and drug sensitivity was dissected. Furthermore, we demonstrated that STK25 knockdown could increase the proliferation, migration, and invasion capacity of HCC cells.
肝细胞癌 (HCC) 是一种侵袭性恶性肿瘤，预后不良。活性氧 (ROS) 在肿瘤中起重要作用；然而，ROS 相关基因在 HCC 中的作用仍不清楚。因此，我们通过生物信息学方法分析了 ROS 相关基因在 HCC 中的作用。首先，使用 LASSO Cox 回归和多变量分析构建了预后模型。我们还研究了 ROS 相关基因的潜在功能及其与免疫浸润、肿瘤干性和药物敏感性的相关性。使用 ICGC 数据库进行验证。其次，我们进一步分析了 11 个 ROS 相关基因在 HCC 中的作用。作为 ROS 基因家族的一员，STK25 在 HCC 中的作用仍不清楚。我们使用实验探索了 STK25 的生物学功能。本研究首次构建了 HCC 中 ROS 相关的预后模型。分析了 ROS 相关基因与免疫浸润、肿瘤干性和药物敏感性的相关性。此外，我们证明 STK25 敲低可以增加 HCC 细胞的增殖、迁移和侵袭能力。
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