Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Research & Development Department, Carlit Holdings Co., Ltd., Gunma, 377-0004, Japan.
Mutat Res Genet Toxicol Environ Mutagen. 2021 Dec;872:503416. doi: 10.1016/j.mrgentox.2021.503416. Epub 2021 Oct 9.
Light emitting diode (LED) devices emit narrow bands of the blue, green, and red light spectrum rather than the continuous spectrum emitted from sunlight and fluorescent light bulbs. LED devices have become considerably common in society, and the fluence of blue light from LED devices is more intense than other light sources. Previous studies presented that the blue light spectrum may harness potentially inimical genotoxicity. Therefore, the aim of this study was to investigate this potential cytotoxicity and genotoxicity, as well as identify the mechanism of the cellular effects induced by blue LED light exposure in mammalian cell lines with their DNA repair deficient mutants. Our results demonstrated that blue LED light induced both oxidative stress to cells and cytotoxic and genotoxic effects including reduction of clonogenicity, cell cycle arrest, induction of sister chromatid exchanges, endoreduplicated chromosomes, and increased frequency of HPRT locus mutations. In DNA repair deficient cells, particularly those involving double strand break repair deficiency, cells presented hypersensitivity to blue LED light exposure. Blue LED light also induced chromosome aberrations more in DNA repair deficient cells than wild type cells. The cytotoxicity of blue LED light was reduced by an effective antioxidant, ascorbic acid 2-glucoside, which can suppress blue LED light induced oxidative stress. These results indicated that prolonged, high intensity exposure to blue LED light induces genotoxic stress to cells, and oxidative stress induced by blue LED light is targeting DNA to induce these biological effects.
发光二极管（LED）设备发射窄带的蓝光、绿光和红光光谱，而不是阳光和荧光灯泡发出的连续光谱。LED 设备在社会中已经相当普遍，并且来自 LED 设备的蓝光辐照度比其他光源更强。先前的研究表明，蓝光光谱可能具有潜在的有害遗传毒性。因此，本研究旨在研究蓝光 LED 照射对哺乳动物细胞系及其 DNA 修复缺陷突变体的潜在细胞毒性和遗传毒性，以及鉴定细胞效应的机制。我们的结果表明，蓝光 LED 既诱导了细胞的氧化应激，又诱导了细胞毒性和遗传毒性效应，包括降低集落形成能力、细胞周期停滞、诱导姐妹染色单体交换、内复制染色体和增加 HPRT 基因座突变频率。在 DNA 修复缺陷细胞中，特别是涉及双链断裂修复缺陷的细胞，对蓝光 LED 照射更为敏感。与野生型细胞相比，蓝光 LED 还会在 DNA 修复缺陷细胞中诱导更多的染色体畸变。有效的抗氧化剂抗坏血酸 2-葡萄糖苷可降低蓝光 LED 诱导的氧化应激，从而降低蓝光 LED 的细胞毒性。这些结果表明，长时间、高强度的蓝光 LED 照射会对细胞产生遗传毒性应激，而蓝光 LED 诱导的氧化应激会靶向 DNA 以诱导这些生物学效应。
