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环磷酸腺苷-环磷酸腺苷受体蛋白（cAMP-CRP）对（某种物质，原文未明确）的调控有助于响应碳饥饿时由鸟苷四磷酸（ppGpp）和鸟苷五磷酸（pppGpp）依赖的积累（某种物质，原文未明确）对葡萄糖耗尽作出反应。 （此译文仅供参考，因原文部分内容缺失或不明确，可能存在理解偏差。）

Regulation of by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation Responds to Glucose Exhaustion.

作者信息

Meyer Laura, Germain Elsa, Maisonneuve Etienne

机构信息

Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-Aix Marseille Univ (UMR7283), Marseille, France.

出版信息

Front Microbiol. 2021 Nov 4;12:775164. doi: 10.3389/fmicb.2021.775164. eCollection 2021.

DOI:10.3389/fmicb.2021.775164
PMID:34803996
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8600398/
Abstract

Guanosine penta- or tetraphosphate (known as (p)ppGpp) serves as second messenger to respond to nutrient downshift and other environmental stresses, a phenomenon called stringent response. Accumulation of (p)ppGpp promotes the coordinated inhibition of macromolecule synthesis, as well as the activation of stress response pathways to cope and adapt to harmful conditions. In , the (p)ppGpp level is tightly regulated by two enzymes, the (p)ppGpp synthetase RelA and the bifunctional synthetase/hydrolase SpoT. We recently identified the small protein YtfK as a key regulator of SpoT-mediated activation of stringent response in . Here, we further characterized the regulation of . We observed that is subjected to catabolite repression and is positively regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Importantly, YtfK contributes to SpoT-dependent accumulation of (p)ppGpp and cell survival in response to glucose starvation. Therefore, regulation of by the cAMP-CRP appears important to adjust (p)ppGpp level and coordinate cellular metabolism in response to glucose availability.

摘要

鸟苷五磷酸或四磷酸（称为(p)ppGpp）作为第二信使来应对营养物质减少和其他环境压力，这一现象称为严紧反应。(p)ppGpp的积累促进了对大分子合成的协同抑制，以及激活应激反应途径以应对和适应有害条件。在[具体生物]中，(p)ppGpp水平由两种酶严格调控，即(p)ppGpp合成酶RelA和双功能合成酶/水解酶SpoT。我们最近鉴定出小蛋白YtfK是[具体生物]中SpoT介导的严紧反应激活的关键调节因子。在此，我们进一步表征了[具体生物]的调控。我们观察到[具体生物]受到分解代谢物阻遏，并且受到环腺苷酸（cAMP）-cAMP受体蛋白（CRP）复合物的正调控。重要的是，YtfK有助于SpoT依赖的(p)ppGpp积累以及细胞在葡萄糖饥饿时的存活。因此，cAMP-CRP对[具体生物]的调控对于调节(p)ppGpp水平和协调细胞代谢以响应葡萄糖可用性似乎很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/c262218f3b02/fmicb-12-775164-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/0c49f775e40f/fmicb-12-775164-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/0f376ed36ea1/fmicb-12-775164-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/92bcbd5ee53f/fmicb-12-775164-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/e2d11dd2d625/fmicb-12-775164-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/6159cf8ed0fd/fmicb-12-775164-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/c262218f3b02/fmicb-12-775164-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/0c49f775e40f/fmicb-12-775164-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/0f376ed36ea1/fmicb-12-775164-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/92bcbd5ee53f/fmicb-12-775164-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/e2d11dd2d625/fmicb-12-775164-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/6159cf8ed0fd/fmicb-12-775164-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bde2/8600398/c262218f3b02/fmicb-12-775164-g006.jpg

相似文献

[1]
Regulation of by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation Responds to Glucose Exhaustion.

Front Microbiol. 2021-11-4

[2]
YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli.

Nat Commun. 2019-12-17

[3]
Rsd balances (p)ppGpp level by stimulating the hydrolase activity of SpoT during carbon source downshift in .

Proc Natl Acad Sci U S A. 2018-6-18

[4]
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[5]
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J Mol Biol. 1996-5-31

[6]
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[7]
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[8]
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[9]
Bacteria possessing two RelA/SpoT-like proteins have evolved a specific stringent response involving the acyl carrier protein-SpoT interaction.

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[10]
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引用本文的文献

[1]
A dual program for CRP-mediated regulation in bacterial alarmone (p)ppGpp.

mBio. 2024-11-13

[2]
(p)ppGpp is required for virulence of .

Infect Immun. 2024-1-16

[3]
Persistence of obligate intracellular pathogens: alternative strategies to overcome host-specific stresses.

Front Cell Infect Microbiol. 2023

[4]
Amino Acid Starvation-Induced Glutamine Accumulation Enhances Pneumococcal Survival.

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本文引用的文献

[1]
The stringent response and physiological roles of (pp)pGpp in bacteria.

Nat Rev Microbiol. 2021-4

[2]
Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel.

Cell Rep. 2020-9-15

[3]
A nucleotide-switch mechanism mediates opposing catalytic activities of Rel enzymes.

Nat Chem Biol. 2020-5-11

[4]
YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli.

Nat Commun. 2019-12-17

[5]
Contributions of SpoT Hydrolase, SpoT Synthetase, and RelA Synthetase to Carbon Source Diauxic Growth Transitions in .

Front Microbiol. 2018-8-3

[6]
Rsd balances (p)ppGpp level by stimulating the hydrolase activity of SpoT during carbon source downshift in .

Proc Natl Acad Sci U S A. 2018-6-18

[7]
Activation of the Stringent Response by Loading of RelA-tRNA Complexes at the Ribosomal A-Site.

Mol Cell. 2018-4-5

[8]
Involvement of the ytfK gene from the PhoB regulon in stationary-phase H2O2 stress tolerance in Escherichia coli.

Microbiology (Reading). 2017-12

[9]
The stringent factor RelA adopts an open conformation on the ribosome to stimulate ppGpp synthesis.

Nucleic Acids Res. 2016-7-27

[10]
The magic dance of the alarmones (p)ppGpp.

Mol Microbiol. 2016-8

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