Lu Run-Lan, Li Ping, Li Bing, Xing Yuan, Zhang Yuan-Yuan, Chen Bin-Ze, Hu Qin-Ni, Yang Zhi-Hua, Gao Bao-Dong, Ha Xiao-Qin, Gao Cai-Yan
Lanzhou Traditional Chinese Medicine Hospital, Lanzhou 730050.
Department of Clinical Laboratory, the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Key Laboratory of Stem Cells and Gene Grug of Gansu Province, Lanzhou 730050, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 Sep;37(5):529-533. doi: 10.12047/j.cjap.6133.2021.074.
To compare the changes in the number of circulating endothelial progenitor cells and hypoxia-inducible factors in patients with type 2 diabetes at different altitudes, and to provide a basis for the research and treatment of type 2 diabetes vascular complications. Selected Type 2 diabetes patients who were diagnosed in a low altitude area of 386 m (Xianyang City) and a high altitude area of 1 520 m (Lanzhou) (25 persons/29 persons) and healthy persons (20 persons/20 persons) were selected. An automatic biochemical analyzer was used to detect the indexes of blood lipids, blood glucose, and glycosylated hemoglobin of the two groups of people, and the concentration of Hypoxia inducible factor-1α (HIF-1α) was detected by enzyme-linked immunosorbent assay (ELISA). The number of circulating endothelial progenitor cells (EPCs) in peripheral blood was determined by a cytometer. No matter in low or high altitude areas, the number of circulating EPCs in the diabetes group was lower than that in the healthy group (＜0.01). The levels of body mass index (BMI), waist to hip ratio (WHR), triglyceride (TG), fasting blood glucose (FBG) and glycosylated hemoglobin (HbAlc) were increased (＜0.05). Compared with the low-altitude group, the expression levels of HIF-1α in diabetic patients at high-altitude and healthy people were increased significantly (＜0.05), while the number of circulating EPCs was decreased significantly (＜0.05), and the number of circulating EPCs in healthy people or the patients with type 2 diabetes without vascular complications was higher than that of patients with type 2 diabetes with vascular complications (＜0.05). With the increase in altitude, the expression level of HIF-1α in type 2 diabetes mellitus(T2DM)patients is increased, and the number of circulating EPCs is decreased, which is closely related to the degree of vascular disease. Therefore, it is possible through transplantation of EPCs for high altitude T2DM patients to achieve the prevention and improvement of diabetic vascular complications.
比较不同海拔地区2型糖尿病患者循环内皮祖细胞数量及缺氧诱导因子的变化，为2型糖尿病血管并发症的研究与治疗提供依据。选取在海拔386米的低海拔地区（咸阳市）和海拔1520米的高海拔地区（兰州市）确诊的2型糖尿病患者（25例/29例）以及健康人（20例/20例）。使用自动生化分析仪检测两组人群的血脂、血糖及糖化血红蛋白指标，采用酶联免疫吸附测定法（ELISA）检测缺氧诱导因子-1α（HIF-1α）浓度。用细胞计数仪测定外周血中循环内皮祖细胞（EPCs）数量。无论在低海拔还是高海拔地区，糖尿病组循环EPCs数量均低于健康组（＜0.01）。体重指数（BMI）、腰臀比（WHR）、甘油三酯（TG）、空腹血糖（FBG）及糖化血红蛋白（HbAlc）水平升高（＜0.05）。与低海拔组相比，高海拔糖尿病患者及健康人中HIF-1α表达水平显著升高（＜0.05），而循环EPCs数量显著减少（＜0.05），且健康人或无血管并发症的2型糖尿病患者循环EPCs数量高于有血管并发症的2型糖尿病患者（＜0.05）。随着海拔升高，2型糖尿病（T2DM）患者HIF-1α表达水平升高，循环EPCs数量减少，这与血管病变程度密切相关。因此，对高海拔T2DM患者进行EPCs移植有可能实现糖尿病血管并发症的预防和改善。
