使用集成在基于侧向流动装置中的寡核苷酸探针进行细菌培养物的超灵敏和特异性检测。

Ultra-Sensitive and Specific Detection of Bacterial Cultures Using an Oligonucleotide Probe Integrated in a Lateral Flow-Based Device.

作者信息

Machado Isabel, Goikoetxea Garazi, Alday Enara, Jiménez Tania, Arias-Moreno Xabier, Hernandez Frank J, Hernandez Luiza I

机构信息

SOMAprobes S.L., Mikeletegi Pasealekua, 83, 20009 Donostia, Spain.

Department of Cellular Biology and Histology, Faculty of Medicine and Odontology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.

出版信息

Diagnostics (Basel). 2021 Oct 31;11(11):2022. doi: 10.3390/diagnostics11112022.

DOI:10.3390/diagnostics11112022

PMID:34829369

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8619029/

Abstract

The identification of pathogens causing infectious diseases is still based on laborious and time-consuming techniques. Therefore, there is an urgent need for the development of novel methods and devices that can considerably reduce detection times, allowing the health professionals to administer the right treatment at the right time. Lateral flow-based systems provide fast, cheap and easy to use alternatives for diagnosis. Herein, we report on a lateral flow approach for specifically detecting bacteria within 6 h.

摘要

传染病病原体的鉴定仍然基于费力且耗时的技术。因此，迫切需要开发能够大幅缩短检测时间的新方法和设备，使医疗专业人员能够在正确的时间给予正确的治疗。基于侧向流动的系统为诊断提供了快速、廉价且易于使用的替代方案。在此，我们报告一种用于在6小时内特异性检测细菌的侧向流动方法。