Canales Celia, Morán Félix, Olmos Antonio, Ruiz-García Ana Belén
Center for Plant Protection and Biotechnology, Valencian Institute of Agricultural Research (IVIA), Ctra. Moncada-Náquera km 4.5, Moncada, 46113 Valencia, Spain.
Plants (Basel). 2021 Oct 25;10(11):2293. doi: 10.3390/plants10112293.
Loquat () is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was investigated. RT-PCR and high-throughput sequencing (HTS) of symptomatic loquat plants led to first-time detection and characterization of apple stem grooving virus (ASGV), also known as citrus tatter leaf virus (CTLV), and apple chlorotic leaf spot virus (ACLSV) from Spain with description of nearly complete genomic sequences. The frequency of ACLSV infection was the highest, with over 30% of the samples testing positive and were also detected as coinfections with ASGV and ASPV, although most of the samples infected were symptomless. Studies on all the full-length sequences available in the databases were performed in order to establish the phylogenetic relationships of the Spanish isolates of these two viral species. Moreover, apple hammerhead viroid (AHVd) was also detected to infect loquat, the first host different from apple reported for this viroid to date.
枇杷（）是西班牙的一种重要作物。迄今为止，在西班牙的枇杷果园中仅检测到一种病毒，即苹果茎痘病毒（ASPV）。在本研究中，调查了西班牙感染这种作物的其他病毒的存在情况。对有症状的枇杷植株进行逆转录聚合酶链反应（RT-PCR）和高通量测序（HTS），首次检测并鉴定了来自西班牙的苹果茎沟病毒（ASGV），也称为柑橘碎叶病毒（CTLV）和苹果褪绿叶斑病毒（ACLSV），并描述了近乎完整的基因组序列。ACLSV感染频率最高，超过30%的样本检测呈阳性，并且还被检测为与ASGV和ASPV的混合感染，尽管大多数受感染样本无症状。对数据库中所有可用的全长序列进行了研究，以建立这两种病毒西班牙分离株的系统发育关系。此外，还检测到苹果锤头状类病毒（AHVd）感染枇杷，这是迄今为止报道的该类病毒不同于苹果的首个宿主。
