Javaran Vahid Jalali, Moffett Peter, Lemoyne Pierre, Xu Dong, Adkar-Purushothama Charith Raj, Fall Mamadou Lamine
Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Jean-sur-Richelieu, QC J3B 3E6, Canada.
Département de Biologie, Centre SÈVE, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.
Plants (Basel). 2021 Oct 31;10(11):2355. doi: 10.3390/plants10112355.
Among all economically important plant species in the world, grapevine ( L.) is the most cultivated fruit plant. It has a significant impact on the economies of many countries through wine and fresh and dried fruit production. In recent years, the grape and wine industry has been facing outbreaks of known and emerging viral diseases across the world. Although high-throughput sequencing (HTS) has been used extensively in grapevine virology, the application and potential of third-generation sequencing have not been explored in understanding grapevine viruses and their impact on the grapevine. Nanopore sequencing, a third-generation technology, can be used for the direct sequencing of both RNA and DNA with minimal infrastructure. Compared to other HTS methods, the MinION nanopore platform is faster and more cost-effective and allows for long-read sequencing. Due to the size of the MinION device, it can be easily carried for field viral disease surveillance. This review article discusses grapevine viruses, the principle of third-generation sequencing platforms, and the application of nanopore sequencing technology in grapevine virus detection, virus-plant interactions, as well as the characterization of viral RNA modifications.
在世界上所有具有重要经济价值的植物物种中，葡萄（Vitis vinifera L.）是种植最为广泛的水果植物。它通过葡萄酒、新鲜水果和干葡萄生产，对许多国家的经济产生了重大影响。近年来，全球葡萄和葡萄酒产业一直面临着已知和新出现的病毒性疾病的爆发。尽管高通量测序（HTS）已在葡萄病毒学中广泛应用，但尚未探索第三代测序在了解葡萄病毒及其对葡萄影响方面的应用和潜力。纳米孔测序作为一种第三代技术，可用于对RNA和DNA进行直接测序，所需基础设施最少。与其他高通量测序方法相比，MinION纳米孔平台速度更快、成本效益更高，并允许进行长读长测序。由于MinION设备体积小，便于携带，可用于田间病毒病监测。本文综述了葡萄病毒、第三代测序平台的原理，以及纳米孔测序技术在葡萄病毒检测、病毒与植物相互作用以及病毒RNA修饰表征方面的应用。
