在出生后晚期角膜发育过程中，胶原 XI 的下调随后在损伤后上调。
Downregulation of collagen XI during late postnatal corneal development is followed by upregulation after injury.
机构信息
From the Cornea, External Disease Service, Department of Ophthalmology, University of South Florida, Tampa, FL 33612, USA.
Research Methodology and Biostatistics Core, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.
出版信息
J Cell Sci. 2022 Jan 1;135(1). doi: 10.1242/jcs.258694. Epub 2022 Jan 12.
Collagen XI plays a role in nucleating collagen fibrils and in controlling fibril diameter. The aim of this research was to elucidate the role that collagen XI plays in corneal fibrillogenesis during development and following injury. The temporal and spatial expression of collagen XI was evaluated in C57BL/6 wild-type mice. For wound-healing studies in adult mice, stromal injuries were created using techniques that avoid caustic chemicals. The temporal expression and spatial localization of collagen XI was studied following injury in a Col11a1 inducible knockout mouse model. We found that collagen XI expression occurs during early maturation and is upregulated after stromal injury in areas of regeneration and remodeling. Abnormal fibrillogenesis with new fibrils of heterogeneous size and shape occurs after injury in a decreased collagen XI matrix. In conclusion, collagen XI is expressed in the stroma during development and following injury in adults, and is a regulator of collagen fibrillogenesis in regenerating corneal tissue.
胶原 XI 在胶原原纤维的成核和纤维直径控制中发挥作用。本研究旨在阐明胶原 XI 在发育过程中和损伤后角膜原纤维形成中的作用。在 C57BL/6 野生型小鼠中评估了胶原 XI 的时空表达。对于成年小鼠的伤口愈合研究，使用避免腐蚀性化学物质的技术来创建基质损伤。在 Col11a1 诱导型敲除小鼠模型中，研究了胶原 XI 的时空表达和空间定位。我们发现，胶原 XI 的表达发生在早期成熟过程中，并在再生和重塑区域的基质损伤后上调。在胶原 XI 基质减少的情况下，损伤后会出现异常的原纤维形成，具有不同大小和形状的新原纤维。总之，胶原 XI 在发育过程中以及成年后受伤时均在基质中表达，是再生角膜组织中胶原原纤维形成的调节剂。
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