Stephenson Maria, Nip Ka Ming, HafezQorani Saber, Gagalova Kristina K, Yang Chen, Warren René L, Birol Inanc
Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, BC V5Z 4S6, Canada.
NAR Genom Bioinform. 2021 Nov 29;3(4):lqab105. doi: 10.1093/nargab/lqab105. eCollection 2021 Dec.
Recent advances in single-cell RNA sequencing technologies have made detection of transcripts in single cells possible. The level of resolution provided by these technologies can be used to study changes in transcript usage across cell populations and help investigate new biology. Here, we introduce RNA-Scoop, an interactive cell cluster and transcriptome visualization tool to analyze transcript usage across cell categories and clusters. The tool allows users to examine differential transcript expression across clusters and investigate how usage of specific transcript expression mechanisms varies across cell groups.
单细胞RNA测序技术的最新进展使得在单细胞中检测转录本成为可能。这些技术提供的分辨率水平可用于研究细胞群体中转录本使用情况的变化,并有助于探索新的生物学现象。在这里,我们介绍RNA-Scoop,这是一种交互式细胞簇和转录组可视化工具,用于分析不同细胞类别和簇中的转录本使用情况。该工具允许用户检查不同簇之间的差异转录本表达,并研究特定转录本表达机制的使用情况在不同细胞组之间是如何变化的。