Johnson K W, Holsapple M P, Munson A E
Fundam Appl Toxicol. 1986 Feb;6(2):317-26.
Various toxicological and immunological parameters were assessed after exposure of female B6C3F1 mice to 0.1, 1.0, 4.0, and 8.0 mg/kg gamma-chlordane for 14 days via oral gavage. Variables evaluated included periodic body weights, terminal organ weights, hematology including leukocyte differentials, antibody response to sheep red blood cells (SRBC), lymphoproliferative responses to the mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and lipopolysaccharide (LPS) and allogeneic cells, and the delayed hypersensitivity response (DHR) to keyhole limpet hemocyanin (KLH). When compared to the corn oil (vehicle) controls, chlordane produced a significant, dose-related increase in liver weight. Total leukocytes were significantly increased in chlordane-treated groups and seemed to be due to a significant increase in the lymphocyte population. Humoral immunity (HI), as assessed by enumeration of SRBC-specific immunoglobulin M (IgM) antibody forming cells (AFC) and proliferation of LPS-stimulated spleen cells, was not significantly altered in mice exposed to chlordane. In vitro evaluation of cell-mediated immunity (CMI), as measured by proliferation of Con A and PHA-stimulated spleen cells from chlordane-treated animals, indicated a significant and dose-related increase. The response to allogeneic cells was also enhanced. Results from an in vivo indicator of cell-mediated immune status, the delayed hypersensitivity response to KLH, did not support chlordane-enhanced T cell function suggested by mitogen and mixed lymphocyte responses. Therefore, a potent cyclodiene insecticide of environmental concern produced an enhancement of certain indicators of CMI. The expression of a DHR in vivo and the antibody response to SRBC was unaltered in mice exposed to the chemical. These results suggest that gamma-chlordane, at the concentrations utilized, does not produce a biologically significant alteration of the immunocompetence of intact animals.
将雌性B6C3F1小鼠通过灌胃给予0.1、1.0、4.0和8.0mg/kg的γ-氯丹，持续14天，之后评估了各种毒理学和免疫学参数。评估的变量包括定期体重、终末器官重量、血液学指标（包括白细胞分类）、对绵羊红细胞（SRBC）的抗体反应、对有丝分裂原植物血凝素（PHA）、刀豆蛋白A（Con A）和脂多糖（LPS）以及同种异体细胞的淋巴细胞增殖反应，以及对钥孔戚血蓝蛋白（KLH）的迟发型超敏反应（DHR）。与玉米油（赋形剂）对照组相比，氯丹使肝脏重量显著增加，且呈剂量相关。氯丹处理组的总白细胞显著增加，似乎是由于淋巴细胞数量显著增加所致。通过计数SRBC特异性免疫球蛋白M（IgM）抗体形成细胞（AFC）和LPS刺激的脾细胞增殖来评估的体液免疫（HI），在接触氯丹的小鼠中没有显著改变。通过氯丹处理动物的Con A和PHA刺激的脾细胞增殖来测量的细胞介导免疫（CMI）的体外评估表明，其显著增加且呈剂量相关。对同种异体细胞的反应也增强了。细胞介导免疫状态的体内指标——对KLH的迟发型超敏反应的结果，并不支持有丝分裂原和混合淋巴细胞反应所表明的氯丹增强T细胞功能。因此，一种受环境关注的强效环二烯类杀虫剂增强了某些CMI指标。接触该化学物质的小鼠体内DHR的表达和对SRBC的抗体反应未改变。这些结果表明，在所使用的浓度下，γ-氯丹不会对完整动物的免疫能力产生生物学上显著的改变。
