Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0028, South Africa.
Department of Zoology and Entomology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0028, South Africa.
Plant Dis. 2022 Jul;106(7):1944-1951. doi: 10.1094/PDIS-09-21-1877-RE. Epub 2022 Jun 13.
Leaf blight caused by is one of the most important diseases of planted in the subtropics and tropics. In contrast, the better-known , though also a primary pathogen of , causes less damage to trees in these areas. Although is an aggressive pathogen, nothing is known about its infection biology. In this study, the conditions for infection and disease development caused by and were evaluated and compared on a × hybrid clone. The optimal temperature for germination ranged from 25 to 30°C for and 15 to 20°C for . The germination of these pathogens was favored under conditions of light and high levels of RH. Penetration by and occurred via stomata, and the hyphae colonized the intercellular spaces of infected leaves. Symptoms were clearly visible 3 weeks after inoculation by both pathogens, and reproductive structures started to develop in substomatal cavities at 4 weeks after inoculation. The results of this study will facilitate the establishment of rapid screening trials based on artificial inoculations aimed at reducing the impact of disease caused by
由 引起的叶枯病是亚热带和热带种植的 最重要的疾病之一。相比之下,更为人熟知的 虽然也是这些地区树木的主要病原体,但对树木造成的损害较小。尽管 是一种侵袭性病原体,但人们对其感染生物学一无所知。在这项研究中,评估并比较了 和 在 × 杂种克隆上引起感染和疾病发展的条件。对于 和 ,萌发的最佳温度范围分别为 25 至 30°C 和 15 至 20°C。这些病原体在光照和高 RH 条件下有利于萌发。 和 通过气孔侵入,菌丝在感染叶片的细胞间隙中定殖。接种后 3 周,两种病原体均可明显观察到症状,接种后 4 周,在亚气孔腔中开始发育繁殖结构。本研究的结果将有助于建立基于人工接种的快速筛选试验,旨在减轻由 引起的疾病的影响。