Puchała M, Schuessler H
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Sep;50(3):535-46. doi: 10.1080/09553008614550931.
Aqueous solutions of haemoglobin were irradiated under N2 and under N2O in the presence of methanol, ethanol and 1-butanol, which were partly 14C-labelled. The amount of bound alcohol was measured after gel filtration on Sephadex G-100 and with sodium dodecyl sulphate on Sepharose 6B-CL. All alcohols became covalently linked to haemoglobin. After radiolysis under N2 the G values for the three different alcohols were very similar, but under N2O the yield of cross-linking of haemoglobin with 1-butanol is twice that of methanol or ethanol. The high degree of modification by butanol results in extensive dissociation to dimers. Possible mechanisms yielding covalent cross-links between alcohols and haemoglobin are discussed. The high G value for cross-linking with butanol suggests that butanol radicals can add very efficiently to double bonds.
在氮气和一氧化二氮氛围下，于甲醇、乙醇和部分用¹⁴C标记的1 - 丁醇存在的条件下，对血红蛋白水溶液进行辐照。在葡聚糖凝胶G - 100上进行凝胶过滤以及在交联琼脂糖6B - CL上用十二烷基硫酸钠处理后，测定结合醇的量。所有醇均与血红蛋白形成共价连接。在氮气氛围下进行辐解后，三种不同醇的G值非常相似，但在一氧化二氮氛围下，血红蛋白与1 - 丁醇的交联产率是甲醇或乙醇的两倍。丁醇的高度修饰导致大量解离为二聚体。文中讨论了醇与血红蛋白之间形成共价交联的可能机制。与丁醇交联的高G值表明丁醇自由基能够非常有效地加成到双键上。
