Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University 1-3 Machikaneyama-Cho, Toyonaka, Osaka 560-8531, Japan.
Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France.
Carbohydr Polym. 2022 Feb 1;277:118820. doi: 10.1016/j.carbpol.2021.118820. Epub 2021 Oct 28.
In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting.
在本研究中，我们制备了一种酚基接枝聚葡萄糖醛酸（PGU），并将其作为生物墨水成分用于挤出式 3D 生物打印中，以评估其在组织工程领域的应用潜力。该 PGU 衍生物是通过与酪胺缀合得到的，其水溶液可通过辣根过氧化物酶（HRP）催化反应实现凝胶化。从 2.0 w/v%、含 5 U/mL HRP 的衍生物溶液中，可成功获得具有良好蓝图形状保真度的水凝胶结构。打印后第 1 天，包埋于构建物中的小鼠成纤维细胞和人肝癌细胞的存活率约为 95%，在 11 天的研究过程中，细胞活力没有明显下降。这些结果表明 PGU 衍生物在组织工程领域具有巨大的应用潜力，特别是作为挤出式 3D 生物打印的生物墨水成分。
