通过免疫荧光法对甘氨酸锌标记物和癌胚抗原进行组织定位。I. 抗甘氨酸锌标记物抗血清的制备。

Tissue localization of zinc glycinate marker and carcinoembryonic antigen by immunofluorescence. I. Preparation of antisera against the zinc glycinate marker.

作者信息

Saravis C A, Oh S K, Pusztaszeri G, Doos W G, Zamcheck N

出版信息

J Natl Cancer Inst. 1978 Jun;60(6):1371-4. doi: 10.1093/jnci/60.6.1371.

DOI:10.1093/jnci/60.6.1371

Abstract

Antisera against the zinc glycinate marker (ZGM) were produced in New Zealand White rabbits with induced tolerance to normal tissue components and CEA, and in mature, previously uninoculated rabbits for use in immunofluorescent histologic localization of ZGM in colon cancers and other tissues. Analysis of the antisera by immunodiffusion and counterimmunoelectrophoretic techniques showed them to be specific for ZGM when tested with ZGM, carcinoembryonic antigen, normal tissue extracts, or cell elements of normal blood.

摘要

针对甘氨酸锌标记物（ZGM）的抗血清是在对正常组织成分和癌胚抗原（CEA）具有诱导耐受性的新西兰白兔中产生的，也在成熟的、先前未接种过的兔子中产生，用于ZGM在结肠癌和其他组织中的免疫荧光组织学定位。通过免疫扩散和对流免疫电泳技术对抗血清进行分析，结果表明，当用ZGM、癌胚抗原、正常组织提取物或正常血液的细胞成分进行检测时，这些抗血清对ZGM具有特异性。