Insight into the generation and consumption mechanism of tightly bound and loosely bound extracellular polymeric substances by mathematical modeling.

The quantity of tightly bound extracellular polymeric substances (TB-EPS) and loosely bound extracellular polymeric substances (LB-EPS) are recognized to be crucial for activated sludge flocculability and settleability. However, the generation and consumption mechanisms of TB-EPS and LB-EPS are vague, and there is no effective model to quantitatively predict LB-EPS and TB-EPS. In this work, a decrease in LB-EPS and TB-EPS was verified to increase the absolute value of the zeta potential and decrease the sludge settling volume, which affects the flocculation and settling performance of sludge. Hence, we comparatively developed, calibrated and validated two different mathematical model structure (named expanded unified model-TL1 and expanded unified model-TL2), aiming to systematically reveal the generation and consumption mechanism of TB-EPS and LB-EPS and quantitatively predict changes of TB-EPS and LB-EPS. On the basis of microbial physiology and the existing literature, two different mechanisms of the generation and consumption of TB-EPS and LB-EPS are described. According to the validation performed, expanded unified model-TL2 fit better with experimental TB-EPS and LB-EPS, which described with the hypotheses: (i) TB-EPS and LB-EPS are simultaneously generated while activate biomass growth on external substrate, (ii) LB-EPS can also be hydrolyzed by TB-EPS, and (iii) Biomass-associated products (BAP) are hydrolyzed by LB-EPS, and it was further proven to be more realistic from the perspective of microbial physiology. This study systematically revealed the generation and consumption mechanism of TB-EPS and LB-EPS by mathematical modeling, and provides a basis for regulating the concentrations of them to improve sludge settling capacity and system stability.