Gyawali Sanjaya, Bhattarai Gehendra, Shi Ainong, Kik Chris, du Toit Lindsey J
Washington State University Mount Vernon Northwestern Washington Research and Extension Center, Mount Vernon, WA, United States.
Department of Horticulture, University of Arkansas, Fayetteville, AR, United States.
Front Genet. 2021 Dec 8;12:740437. doi: 10.3389/fgene.2021.740437. eCollection 2021.
Genotype-by-sequencing (GBS) was used to explore the genetic diversity and structure of , and the selective sweeps involved in domestication of cultivated spinach, , from . A total 7,065 single nucleotide polymorphisms (SNPs) generated for 16 and 76 accessions placed the accessions in one group, 1, and the 76 accessions, which originated from Central Asia, in two distinct groups, 2 and 3. The 2 group shared greater genetic identity with the accessions, 1, than the 3 group. Likewise, the 1 group had a smaller st (0.008) with the 2 group than with the 3 group (st = 0.012), and a greater gene flow (Nm = 30.13) with the 2 group than with the 3 group (Nm = 21.83). The 2 accessions originated primarily from Uzbekistan while the 3 accessions originated mostly from Tajikistan. The Zarafshan Mountain Range appears to have served as a physical barrier that largely separated members of the 2 and 3 groups of . Accessions with admixtures of 2 and 3 were collected primarily from lower elevations at the southern end of the Zarafshan Mountain Range in Uzbekistan. Selective sweep regions identified at 32, 49, and 52 Mb on chromosomes 1, 2, and 3, respectively, appear to have played a vital role in the domestication of as they are correlated with important domestication traits, including day length sensitivity for bolting (flowering). High XP-CLR scores at the 52 Mb genomic region of chromosome three suggest that a selective sweep at this region was responsible for early differentiation of into two groups in Central Asia.
通过测序进行基因型分析（GBS）被用于探究栽培菠菜（Spinacia oleracea L.）的遗传多样性和结构，以及其从野生菠菜（Spinacia turkestanica Iljin）驯化过程中涉及的选择性清除。总共为16份栽培菠菜和76份野生菠菜材料生成了7065个单核苷酸多态性（SNP），这些栽培菠菜材料聚为一组（组1），而76份源自中亚的野生菠菜材料分为两个不同的组（组2和组3）。组2的野生菠菜材料与组1的栽培菠菜材料相比，具有更高的遗传相似性。同样，组1与组2的固定指数（Fst = 0.008）小于其与组3的固定指数（Fst = 0.012），且与组2的基因流（Nm = 30.13）大于其与组3的基因流（Nm = 21.83）。组2的野生菠菜材料主要源自乌兹别克斯坦，而组3的野生菠菜材料大多源自塔吉克斯坦。泽拉夫善山脉似乎起到了物理屏障的作用，在很大程度上分隔了野生菠菜的组2和组3成员。同时含有组2和组3成分的材料主要采集自乌兹别克斯坦泽拉夫善山脉南端的低海拔地区。分别在1号、2号和3号染色体上32、49和52 Mb处鉴定出的选择性清除区域，似乎在菠菜驯化过程中发挥了至关重要的作用，因为它们与重要的驯化性状相关，包括抽薹（开花）的日长敏感性。3号染色体52 Mb基因组区域的高XP-CLR分数表明，该区域的选择性清除导致了中亚地区野生菠菜早期分化为两个组。
