克罗地亚葡萄藤病毒H的检测、传播及特性分析

Detection, Transmission, and Characterization of Grapevine Virus H in Croatia.

作者信息

Jagunić Martin, Lazarević Boris, Nikolić Kristina, Stupić Domagoj, Preiner Darko, Vončina Darko

机构信息

Department of Plant Pathology, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia.

Department of Plant Nutrition, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia.

出版信息

Pathogens. 2021 Dec 3;10(12):1578. doi: 10.3390/pathogens10121578.

DOI:10.3390/pathogens10121578

PMID:34959533

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8704696/

Abstract

A survey of recently discovered vitiviruses was performed on 113 Croatian autochthonous grapevine cultivars from the national collection "Jazbina" using one-step RT-PCR. The presence of grapevine virus H (GVH) was confirmed in nine (7.9%) cultivars and grapevine virus G in eight (7.1%), while the presence of grapevine viruses I and J were not detected. GVH was transmitted by the vine mealybug () from a source plant to grapevine seedlings with a 10.5% transmission rate using a combination of 10 first and second instars per plant with 48 and 72 h of acquisition and inoculation access period, respectively. Transmission correlated with the presence of grapevine leafroll-associated virus 3 (GLRaV-3) in the GVH-source plant and recipient seedlings. No alternative GVH host was identified. A comparison of 356 nt fragments of the RdRP and CP coding regions showed nucleotide identity between the Croatian GVH isolates in the range of 95.5-99.2% and 97.5-99.4% and amino acid identity between 95.8 and 100% and between 98.3 and 100%, respectively. Comparison with foreign isolates revealed nucleotide sequence similarity in the RdRP and CP between 94 and 100% and between 97.7-100%, respectively. To the best of our knowledge, this is the first report of GVH in Croatia and the first identification of the vine mealybug as a vector of GVH.

摘要

使用一步法逆转录聚合酶链反应（RT-PCR），对来自国家葡萄品种收集库“Jazbina”的113个克罗地亚本土葡萄品种进行了近期发现的葡萄病毒调查。在9个（7.9%）品种中确认存在葡萄病毒H（GVH），在8个（7.1%）品种中确认存在葡萄病毒G，而未检测到葡萄病毒I和J。使用每株植物10只一龄和二龄若虫的组合，分别经过48小时和72小时的获取期和接种期，GVH通过葡萄粉蚧从源植物传播到葡萄幼苗，传播率为10.5%。传播与GVH源植物和受体幼苗中葡萄卷叶相关病毒3（GLRaV-3）的存在相关。未鉴定出替代的GVH宿主。对RNA依赖的RNA聚合酶（RdRP）和衣壳蛋白（CP）编码区356个核苷酸片段的比较显示，克罗地亚GVH分离株之间的核苷酸同一性在95.5 - 99.2%和97.5 - 99.4%之间，氨基酸同一性分别在95.8%至100%和98.3%至100%之间。与国外分离株的比较显示，RdRP和CP中的核苷酸序列相似性分别在94%至100%和97.7%至100%之间。据我们所知，这是克罗地亚关于GVH的首次报告，也是首次鉴定出葡萄粉蚧为GVH的传播媒介。