Tan Shimeng, Chen Yanying, Zhou Guoying, Liu Junang
Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, China.
Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China.
Plants (Basel). 2021 Dec 5;10(12):2672. doi: 10.3390/plants10122672.
Anthracnose, caused by spp., is a significant disease affecting oil tea ( Abel.). Extensive molecular studies have demonstrated that is the dominant pathogen of oil tea anthracnose in China. This study aims to investigate differences in molecular processes and regulatory genes at a late stage of infection of , to aid in understanding differences in pathogenic mechanisms of of different geographic populations. We compared the pathogenicity of from different populations (Wuzhishan, Hainan province, and Shaoyang, Hunan province) and gene expression of representative strains of the two populations before and after inoculation in oil tea using RNA sequencing. The results revealed that from Wuzhishan has a more vital ability to impact oil tea leaf tissue. Following infection with oil tea leaves, up-regulated genes in the strains from two geographic populations were associated with galactosidase activity, glutamine family amino acid metabolism, arginine, and proline metabolism. Additionally, up-regulated gene lists associated with infection by Wuzhishan strains were significantly enriched in purine metabolism pathways, while Shaoyang strains were not. These results indicate that more transcriptional and translational activity and the greater regulation of the purine metabolism pathway in the of the Wuzhishan strain might contribute to its stronger pathogenicity.
炭疽病由[具体病原菌名称]引起，是影响油茶（[油茶学名]）的一种重要病害。广泛的分子研究表明，[具体病原菌名称]是中国油茶炭疽病的主要病原菌。本研究旨在调查[具体病原菌名称]在感染后期分子过程和调控基因的差异，以帮助理解不同地理种群的[具体病原菌名称]致病机制的差异。我们比较了来自不同种群（海南省五指山和湖南省邵阳）的[具体病原菌名称]的致病性，以及使用RNA测序技术对油茶接种前后这两个种群代表性菌株的基因表达情况。结果显示，来自五指山的[具体病原菌名称]对油茶叶片组织的影响能力更强。在用油茶叶片感染后，两个地理种群菌株中上调的基因与半乳糖苷酶活性、谷氨酰胺家族氨基酸代谢、精氨酸和脯氨酸代谢相关。此外，与五指山菌株感染相关的上调基因列表在嘌呤代谢途径中显著富集，而邵阳菌株则没有。这些结果表明，五指山菌株的[具体病原菌名称]中更多的转录和翻译活性以及对嘌呤代谢途径的更大调控可能导致其更强的致病性。
