Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California, USA.
Rocky Mountain Biological Laboratory, Crested Butte, Colorado, USA.
Glob Chang Biol. 2022 Apr;28(7):2491-2504. doi: 10.1111/gcb.16064. Epub 2022 Jan 20.
Mapping geographic mosaics of genetic variation and their consequences via genotype x environment interactions at large extents and high resolution has been limited by the scalability of DNA sequencing. Here, we address this challenge for cytotype (chromosome copy number) variation in quaking aspen, a drought-impacted foundation tree species. We integrate airborne imaging spectroscopy data with ground-based DNA sequencing data and canopy damage data in 391 km of southwestern Colorado. We show that (1) aspen cover and cytotype can be remotely sensed at 1 m spatial resolution, (2) the geographic mosaic of cytotypes is heterogeneous and interdigitated, (3) triploids have higher leaf nitrogen, canopy water content, and carbon isotope shifts (δ C) than diploids, and (4) canopy damage varies among cytotypes and depends on interactions with topography, canopy height, and trait variables. Triploids are at higher risk in hotter and drier conditions.
通过基因型与环境相互作用在大范围内和高分辨率上绘制遗传变异的地理镶嵌图，一直受到 DNA 测序可扩展性的限制。在这里，我们解决了震颤白杨（一种受干旱影响的基础树种）的细胞型（染色体拷贝数）变异的这一挑战。我们整合了来自科罗拉多州西南部 391 公里范围内的航空成像光谱数据以及地面 DNA 测序数据和树冠损伤数据。我们表明：(1) 白杨覆盖率和细胞型可以在 1 米的空间分辨率下进行遥感；(2) 细胞型的地理镶嵌是不均匀和交织的；(3) 三倍体的叶片氮含量、冠层含水量和碳同位素位移（δC）都高于二倍体；(4) 树冠损伤在不同的细胞型之间存在差异，并且取决于与地形、冠层高度和性状变量的相互作用。在更热和更干燥的条件下，三倍体面临更高的风险。
