Ron Y, Isakov N, Sprent J
J Immunol. 1987 Jul 15;139(2):400-5.
MRL/lpr mice were used as a model for seeking information on the role of B cells as antigen-presenting cells in vivo. In confirmation of the finding of other workers, MRL/lpr mice with pronounced lymphadenopathy failed to undergo T cell priming in lymph nodes (LN) after s.c. injection of keyhole limpet hemocyanin (KLH) in complete Freund's adjuvant (CFA): thus, in contrast to normal mice or young MRL/lpr mice, the LN cells from older MRL/lpr mice failed to give secondary T proliferative responses to the injected antigen in vitro. Because previous work from this laboratory has shown that B cells play a major role in priming T cells in LN of normal mice, we tested the possibility that the lack of T cell priming to KLH seen in older MRL/lpr LN reflected the relative paucity of B cells. To test this idea, MRL/LPR mice were injected s.c. with B cells taken from normal or young MRL/lpr mice 1 day before priming with KLH/CFA. In the case of old (20 to 24 wk) MRL/lpr mice with massive lymphadenopathy, prior injection of B cells had virtually no effect in promoting LN priming. In marked contrast, injection of B cells into mice exhibiting less-pronounced LN enlargement (mice tested at 14 to 16 wk) was highly effective in restoring LN priming; before B cell injection, the LN of these mice contained only 2 to 5% B cells. These findings add to the evidence that T cell priming in LN is heavily dependent on the presence of B cells.
MRL/lpr小鼠被用作一种模型，以探寻B细胞作为体内抗原呈递细胞的作用相关信息。为证实其他研究人员的发现，患有明显淋巴结病的MRL/lpr小鼠在皮下注射完全弗氏佐剂（CFA）中的钥孔戚血蓝蛋白（KLH）后，未能在淋巴结（LN）中引发T细胞致敏：因此，与正常小鼠或年轻的MRL/lpr小鼠不同，来自老年MRL/lpr小鼠的LN细胞在体外对注射的抗原未能产生次级T增殖反应。由于本实验室先前的研究表明B细胞在正常小鼠的LN中引发T细胞方面起主要作用，我们测试了老年MRL/lpr LN中缺乏对KLH的T细胞致敏反映B细胞相对缺乏的可能性。为验证这一想法，在以KLH/CFA致敏前1天，给MRL/LPR小鼠皮下注射取自正常或年轻MRL/lpr小鼠的B细胞。对于患有大量淋巴结病的老年（20至24周）MRL/lpr小鼠，预先注射B细胞对促进LN致敏几乎没有作用。与之形成鲜明对比的是，将B细胞注射到LN肿大不太明显的小鼠（14至16周龄的小鼠）中，在恢复LN致敏方面非常有效；在注射B细胞之前，这些小鼠的LN中仅含有2%至5%的B细胞。这些发现进一步证明LN中的T细胞致敏严重依赖于B细胞的存在。
