Johnson W T, Kramer T R
J Nutr. 1987 Jun;117(6):1085-90. doi: 10.1093/jn/117.6.1085.
The effects of copper deficiency on the proteins of rat erythrocyte membranes were assessed by electrophoretic analysis. For 42 d, rats were fed diets containing less than 1 ppm Cu with 35 or 250 ppm Fe or 5 ppm Cu with 35 or 250 ppm Fe. Electrophoresis of erythrocyte membrane proteins indicated a significant increase in the amount of a 170,000-dalton protein in rats fed copper-deficient diets. High dietary iron reduced the amount of the 170,000-dalton protein in erythrocyte membranes from rats in both the copper-deficient and copper-adequate groups. However, feeding high dietary iron to copper-deficient rats did not reduce the amount of the protein to the level found in rats fed the copper-adequate diet containing 35 ppm Fe. Triton X-100 extraction of erythrocyte membranes demonstrated that the 170,000-dalton protein was associated with the membrane cytoskeleton. Thus, copper deficiency possibly alters the cell's mechanical properties and consequently decreases erythrocyte survivability by modifying the membrane cytoskeleton.
通过电泳分析评估了铜缺乏对大鼠红细胞膜蛋白的影响。将大鼠分别喂食含铜量低于1 ppm且含铁量为35或250 ppm的饲料，或含铜量为5 ppm且含铁量为35或250 ppm的饲料，持续42天。红细胞膜蛋白的电泳结果表明，喂食缺铜饲料的大鼠中，一种170,000道尔顿的蛋白含量显著增加。高剂量的膳食铁降低了缺铜组和铜充足组大鼠红细胞膜中170,000道尔顿蛋白的含量。然而，给缺铜大鼠喂食高剂量膳食铁并没有使该蛋白的含量降低到喂食含35 ppm铁的铜充足饲料的大鼠中的水平。用Triton X - 100提取红细胞膜表明，170,000道尔顿的蛋白与膜细胞骨架相关。因此，铜缺乏可能会改变细胞的机械性能，进而通过修饰膜细胞骨架降低红细胞的存活率。
